巧酸霉饲料添加剂中丙三醇的测定

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范志先， 武智强， 赵文英， 孙素莉， 王朋涛

（青岛科技大学 化工学院，山东 青岛 266042）

 摘要： 采用气相色谱氢火焰离子化检测器（GCFID）建立巧酸霉饲料添加剂中微量丙三醇的内标定量分析方法。选用DBWAX（30 m×025 mm×025 μm）毛细管色谱柱，二乙二醇为内标物对丙三醇进行测定。结果表明：在20 ~ 1 000 mg·L -1浓度范围内，丙三醇的线性方程为Y =9138 9X - 0070 7，R=0999 9；067%和67% 2个质量分数添加水平的平均回收率分别为886%和1058%；样品8次重复测定的平均值为056%，相对标准偏差（RSD）为252%。

 关键词： 饲料添加剂； 丙三醇； 气相色谱； 内标法

 中图分类号： O 657.7          文献标志码： A

 Determination of Glycerin in Qiaosuanmei Feed Additive

 FAN Zhixian, WU Zhiqiang, ZHAO Wenying, SUN Suli, WANG Pengtao

 (CollegeofChemical Engineering,QingdaoUniversityofScienceand Technology,Qingdao   266042,China)

 Abstract: The internal standard quantitative analysis method of glycerin in a Qiaosuanmei feed additive was established by GCFID. Glycerin was separated with DBWAX（30 m×025 mm×025 μm）capillary column and determined with diethylene glycol as internal standard substance. The result showed that among the 20—1 000 mg·L -1 concentration range, the liner equation was Y= 9138 9 X - 0070 7, R=0999 9. The average recovery of two mass concentration fortification levels of 067% and 67% were 886% and 1058%, respectively. By measuring 8 parallel sample, the mean value was obtained as 056%, and the RSD was 252%.

 Key words: feed additive; glycerin; gas chromatography; internal standard method

 收稿日期： 20150119

作者简介： 范志先(1957—)，男，教授.

文章编号： 16726987（2016）02014303； DOI： 10.16351/j.16726987.2016.02.005