柚皮总黄酮提取工艺的响应面法优化及其抗氧化活性研究

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公衍玲，  刘菲

 （青岛科技大学 化工学院，山东 青岛 266042）

 摘要： 采用乙醇回流法提取柚皮中总黄酮，研究其最佳工艺条件及其抗氧化活性。在单因素实验基础上，选择液料比、乙醇浓度、回流时间3因素，采用星点设计响应面法优化提取工艺条件。采用邻苯三酚法测定柚皮提取液的体外抗氧化活性。星点实验结合生产实际，乙醇回流提取的最佳工艺条件为液料比20∶1（mL∶g），乙醇浓度76%，回流时间95 min，回流温度90 ℃，提取2次。该条件下总黄酮得率预测值为1032%，实测平均值为1036%，RSD为072%。柚皮提取液具有良好的抗氧化活性，并呈现出一定的剂量依赖关系。

关键词： 柚皮； 总黄酮； 提取； 响应面法； 抗氧化活性

 中图分类号： R 284.2       文献标志码： A

Optimization of Extraction Conditions of Total Flavonoids from Pomelo Peel Using Response Surface Methodology and  Its Antioxidant Activity

 GONG Yanling, LIU Fei

 (CollegeofChemical Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)

 Abstract: Total flavonoids from pomelo peel were extracted by ethanol reflux extraction, and the optimal conditions for extraction and its antioxidant activity were determined in this experiment. Three extraction parameters including liquidtosolid ratio, ethanol concentration and reflux time were identified as main factors affecting the extraction yield of total flavonoids. The extraction parameters were optimized by a central composite designresponse surface methodology (CCDRSM). Antioxidant activity of pomelo peel extract was measured by Pyrogallol method in vitro. The results combined with practice revealed that the optimal conditions for total flavonoids extraction from pomelo peel were 95 min extraction twice with 76% ethanol at a liquidtosolid ratio of 20∶1（mL∶g） and reflux temperature of 90 ℃. Under these conditions, the predicted and experimental extraction yields of total flavonoids were 1032% and 1036% respectively and RSD was 072%. The antioxidant activity in vitro experiments showed that pomelo peel extract had good antioxidant activity, and exhibited a certain dosedependent relationship.

 Key words: pomelo peel; total flavonoids; extraction; response surface methodology; antioxidant activity

收稿日期： 20141016

作者简介： 公衍玲（1975—），女，副教授.

文章编号： 16726987（2016）02013706； DOI： 10.16351/j.16726987.2016.02.004