大孔树脂法纯化刺玫叶总黄酮工艺

PDF全文下载：2015060622

王晓林， 钟方丽， 谷丽娟， 徐欣

（吉林化工学院 化学与制药工程学院，吉林 吉林 132022）

摘要： 建立了大孔树脂纯化刺玫叶总黄酮的最佳工艺条件。以吸附率、解吸率为主要考察指标，采用静态吸附及解吸、动态吸附及解吸实验，确定了LX36型大孔树脂纯化刺玫叶总黄酮的效果较理想，并对其纯化条件进行了优化。其最佳纯化条件：上样量为m（生药）∶m（树脂）=1∶25，吸附速率为2 BV·h-1，静置15 min，吸附后用2 BV蒸馏水洗脱，再用5 BV的70%乙醇以3 BV·h-1的流速洗脱，在此最佳条件下，其吸附率平均可为9424%，解吸率平均为8859%，纯化后刺玫叶干浸膏中总黄酮含量由原来的821%提高到2512%，树脂富集倍数约为3倍。结果表明，采用LX36型大孔树脂对刺玫叶总黄酮的纯化具有一定效果。

关键词： 刺玫叶； 大孔树脂； 黄酮； 纯化

中图分类号： R 284.2            文献标志码： A

 Purification Technology of Total Flavonoids in Leaves of Rosa Davurica Pall.with Macroporous Resin Method

WANG Xiaolin, ZHONG Fangli, GU Lijuan, XU Xin

 (School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022,China)

Abstract: The purification technology of total flavonoids(TF) in leaves of Rosa davurica Pall. with macroporous resin was optimized. Applying both static and dynamic absorptiondesorption experiments, the optimum purification conditions with LX36 macroporous resin which offered ideal purification function were determined using absorption ratio and desorption ratio of TS as evaluating indicators. The loading quantity of sample m(crude drugs)∶m(resin), adsorption rate, adsorption time, volume of water, ethanol content, desorption rate, the volume of eluant were 1∶25, 2 BV·h-1, 15 min，2 BV，70%, 3 BV·h-1, and 5 BV respectively, the average absorption and desorption rate were 9424% and 8859%. The purity of TF in extracts could be changed from 821% to 2512%, which offered about 3 times enrichment with this type of resins. The experimental results have shown that LX36 macroporous resin was fit for the purification of TS in leaves of Rosa davurica Pall.

 Key words: leaves of Rosa davurica Pall.; macroporous absorption resin; flavonoids; purification

收稿日期： 20141201

作者简介： 王晓林（1969—），男，副教授.

文章编号： 16726987（2015）06062206； DOI： 10.16351/j.16726987.2015.06.006