一株海洋芽孢杆菌对亚甲基蓝的吸附作用

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文章编号： 1672-6987（2024）02-0015-08； DOI： 10.16351/j.1672-6987.2024.02.002

徐湘薇1a,1b,2,3， 张继福4， 张云1a,1b,1c,2， 胡云峰1a,1b,2*(1.中国科学院南海海洋研究所 a.中国科学院热带海洋生物资源与生态重点实验室; b.广东省海洋药物重点实验室;

c.仪器设备公共服务中心， 广东 广州 510301； 2. 南方海洋科学与工程广东省实验室（广州）， 广东 广州 511458；

3. 中国科学院大学 地球与行星科学学院， 北京 100049； 4. 广东省中医院 针灸科， 广东 广州 510120)

摘要： 从深海环境样品中分离并筛选出了一株对亚甲基蓝染料具有较高吸附能力的芽孢杆菌Bacillus sp. LM-24，优化了其吸附条件并选用硅胶和大孔树脂两种载体对其进行固定化。3种吸附剂最适吸附剂浓度分别为1216、3、10 g·L-1；最佳pH分别为90、90、80；最适吸附温度分别为30、25、25 ℃，最佳吸附时间分别为05、1、2 h，对10 mg·L-1亚甲基蓝去除率分别为88%、90%、99%。动力学与等温线实验表明：3种吸附剂均存在物理与化学吸附，固定化吸附剂表面为非匀相吸附。固定化LM-24的吸附性能要优于游离微生物LM-24，对亚甲基蓝溶液具有快速且稳定的吸附作用，可作为偶氮脱色染料的潜在菌株。

关键词： 海洋芽孢杆菌； 固定化； 吸附； 亚甲基蓝

中图分类号： O 647.33文献标志码： A

引用格式： 徐湘薇， 张继福， 张云， 等. 一株海洋芽孢杆菌对亚甲基蓝的吸附作用［J］. 青岛科技大学学报(自然科学版), 2024, 45（2）： 15-28.

XU Xiangwei， ZHANG Jifu， ZHANG Yun，et al. Adsorption of methylene blue by one marine bacillus strain［J］. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2024， 45（2）： 15-28.

Adsorption of Methylene Blue by One Marine Bacillus Strain

XU Xiangwei1a,1b,2,4，ZHANG Jifu4， ZHANG Yun1a,1b,1c,2， HU Yunfeng1a,1b,2

(1. a.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology; b. Guangdong Key Laboratory of Marine Materia Medical;

c. Equipment Public Service Center, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China；

2. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China；

3. College of Earth and Planetary Science, University of Chinese Academy of Sciences, Beijing 100049, China；

4. Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China)

Abstract: Bacillus sp. LM-24 with high adsorption capacity for methylene blue was isolated and screened from deep-sea environmental samples. Its adsorption conditions were optimized and immobilized with silica gel and macroporous resin. The optimum concentrations of the three adsorbents were 1216,3 and 10 g·L-1 respectively. The optimum pH was 90, 90 and 85 respectively. The optimum adsorption temperatures were 30, 25 and 25 ℃, and the optimum adsorption times were 05, 1 and 4 h respectively. The removal rates of 10 mg·L-1 methylene blue were 88%, 90% and 99% respectively. Kinetic and isotherm experiments show that the three adsorbents have physical and chemical adsorption, and the surface of the immobilized adsorbent is heterogeneous adsorption. The stability of immobilized Bacillus sp. LM-24 has improved significantly in acid conditions and high concentration dyes, and it has rapid and stable adsorption on methylene blue solution. The strain can be used as a potential strain for decolorizing azo dyes.

Key words: Marine bacillus; immobilization; adsorbent; methylene blue

收稿日期： 2023-02-21

基金项目： 国家自然科学基金项目（21302199）；南方海洋科学与工程广东省实验室（广州）人才团队引进重大专项项目（GML2019ZD0406）.

作者简介： 徐湘薇（1995—），女，硕士研究生.*通信联系人.