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基于变性泡介导的加速链交换扩增技术在新型冠状病毒核酸检测中的性能分析

作者:时间:2024-04-19点击数:


全文下载: 202402003.pdf


文章编号: 1672-6987202402-0023-06 DOI 10.16351/j.1672-6987.2024.02.003


陈磊1 赵晓丽2 潘兆宝1 程娟1 王怀杰1 曲梅花1 石超3 杨国儒1* 马翠萍2*1.潍坊市第二人民医院 检验科; 转化医学中心; 呼吸与危重症医学科, 山东 潍坊 261041

2.青岛科技大学 教育部生命科学光电传感与分析化学重点实验室; 山东省生化工程重点实验室:

海洋科学与生物工程学院;青岛核酸快速检测工程研究中心, 山东 青岛 266042

3.青岛大学 生命科学学院; 核酸快速检测国际科技合作基地; 基础医学院; 附属医院临床检验科, 山东 青岛 266071


摘要: 运用变性泡介导的加速链交换扩增技术,建立一种新型冠状病毒(SARS-CoV-2)核酸检测的新方法。收集潍坊市第二人民医院检验科1 492例疑似SARS-CoV-2 样本,以荧光定量PCR结果为参考,计算ASEA-SARS-CoV-2核酸检测试剂盒检测新型冠状病毒样本结果的敏感性、特异性、阳性预测值(PPV)、阴性预测值(NPV)。结果表明:ASEA可在30 min内完成核酸扩增,检测限为1 000拷贝·mL-1。敏感性为967%,特异性为999%。该方法可在30 min内检测SARS-CoV-2,而之前报道的传统qPCR方法需要数小时。建立了一种基于变性泡介导的加速链交换扩增技术检测新型冠状病毒的新方法。


关键词: 加速链交换扩增; 新型冠状病毒; 核酸检测; RNA检测


中图分类号: R 37文献标志码: A

引用格式: 陈磊, 赵晓丽, 潘兆宝, . 基于变性泡介导的加速链交换扩增技术在新型冠状病毒核酸检测中的性能分析[J. 青岛科技大学学报(自然科学版), 2024, 452): 23-28.


CHEN Lei, ZHAO Xiaoli, PAN Zhaobao, et al. A rapid nucleic acid test of SARS-CoV-2 based on accelerated strand exchange amplificationJ. Journal of Qingdao University of Science and TechnologyNatural Science Edition), 2024 452): 23-28.


A Rapid Nucleic Acid Test of SARS-CoV-2 Based on Accelerated

Strand Exchange Amplification


CHEN Lei1, ZHAO Xiaoli2, PAN Zhaobao1, CHENG Juan1, WANG Huaijie1,

QU Meihua1, SHI Chao3, YANG Guoru1, MA Cuiping2

(1Department of Laboratory Medicine; Translational Medical Center; Departemtn of Pulmonary and Critical Care

Medicine, Weifang Second People′s Hospital, Weifang 261041, China

2Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science; MOE, Shandong Provincial

Key Laboratory of Biochemical Engineering; College of Marine Science and Biological Engineering; Qingdao Nucleic

Acid Rapid Detection Engineering Research Center Qingdao University of Science and Technology, Qingdao 266042, China

3College of Life Sciences; Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base;

Department of Pathogenic Biology; School of Basic Medicine; The Affiliated Hospital Qingdao University, Qingdao 266071, China


Abstract: Using the technique ofaccelerated strand exchange amplification (ASEA), a new method for the detection of novel coronavirus nucleic acid was established. Retrospective analysis was performed on 1 492 samples from the laboratory department of Weifang Second People′s hospital. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and agreement rate of the detection results of the SARS-CoV-2 nucleic acid detection kit were calculated. The nucleic acid detection kit could complete nucleic acid amplification within 30 min with a detection limit of 2 copies per reaction. Among the 1492 samples from patients with respiratory tract infections respiratory tract infections, the sensitivity of this kit was 967%, and the specificity was 999%. This kit for detecting SARS-CoV-2 within only 30 min, while the previously reported traditional qPCR method took several hours. We have proved a rapid nucleic acid detection kit for detecting SARS-CoV-2 with accelerating strand exchange amplification.


Key words: accelerated strand exchange amplification; SARS-CoV-2; nucleic acid; RNA detection


收稿日期: 2023-06-15

基金项目: 国家重点研发计划项目(2018YFE0113300; 山东省自然科学基金重点项目(ZR2020KH030; 潍坊市卫健委科技项目(WFWSJK-2020-063; WFWSJK-2020-004; WFWSJK-2020-077; 潍坊市2020年新冠病毒疫情防控专项项目(2020YQFK017.

作者简介: 陈磊(1990—),男,检验师. 同等贡献.*通信联系人.






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