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202105004.pdf
文章编号： 1672-6987（2021）05-0024-06； DOI： 10.16351/j.1672-6987.2021.05.004
王高敏， 吴越， 王霞， 陈夫山， 吉喆*（青岛科技大学 海洋科学与生物工程学院， 山东 青岛 266042）
摘要： 芹菜素是芹菜中重要的活性化合物，具有抗癌、抗氧化、降血压的药用价值。传统的提取方法耗能高效率低，而超临界CO2流体萃取法可以有效的将芹菜素与其结构类似物分离，近年来在中草药活性成分的提取领域有重要应用。本研究使用CO2配以95%、99%浓度的乙醇为辅溶剂对芹菜中的芹菜素进行萃取，并通过高效液相色谱（HPLC）对其含量进行测定，以探究超临界萃取芹菜素的最佳条件。结果表明：添加辅溶剂乙醇可以显著提高芹菜素的萃取率，当辅溶剂乙醇浓度为95%时芹菜素的萃取率最高，可达0006%。在整个萃取过程中，前120 min萃取效率最高，约有90%的芹菜素被萃取出来，当萃取时间持续到150 min时，芹菜素已基本萃取完全。
关键词： 芹菜素； 超临界流体萃取（SFE）； 高效液相色谱（HPLC）； 乙醇浓度； 定量分析
中图分类号： TS 218文献标志码： A
引用格式： 王高敏， 吴越， 王霞， 等. 超临界流体-高效液相色谱法萃取芹菜中芹菜素［J］. 青岛科技大学学报(自然科学版), 2021, 42(5): 24-29.
WANG Gaomin, WU Yue, WANG Xia, et al. Supercritical fluid-high performance liquid chromatography extraction of apigenin from celery［J］. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2021， 42（5）： 24-29.
Extraction of Apigenin from CeleryWANG Gaomin, WU Yue, WANG Xia, CHEN Fushan, JI Zhe
(College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: Apigenin is an important active compound in celery which has significant medicinal value. In this study, the supercritical fluid extraction method was used to extract apigenin from celery using carbon dioxide and ethanol with 95% and 99% concentration as the auxiliary solvent, and its content was determined by high performance liquid chromatography (HPLC) to explore the optimal conditions for supercritical extraction of apigenin. The results showed that the extraction rate of apigenin could be significantly increased by adding cosolvent ethanol, and the extraction rate of apigenin was the highest when the concentration of cosolvent ethanol was 95%, up to 0006%. In the whole extraction process, the extraction efficiency was the highest in the first 120 min, about 90% apigenin was extracted. When the extraction time lasted to 150 min, apigenin was basically extracted completely.
Key words: apigenin; supercritical fluid extraction (SFE); high performance liquid chromatography extraction (HPLC); ethanol concentration; quantitative analysis
收稿日期： 2020-11-24
基金项目： 国家自然科学基金项目（32001277，22078167）；山东省重点研发计划项目（2019JZZY020213）.
作者简介： 王高敏(1996—),女，硕士研究生.*通信联系人.
Supercritical Fluid-High Performance Liquid Chromatography
