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不同CO2浓度对普通小球藻生化组成及光合生理的影响

作者:时间:2020-06-15点击数:

全文下载:  202003003.pdf

 

文章编号: 1672-6987(2020)03-0028-07; DOI: 10.16351/j.1672-6987.2020.03.003

 

梁成伟, 王璐, 石蕾

(青岛科技大学 海洋科学与生物工程学院,山东 青岛 266042)

 

摘要: 探讨了普通小球藻对于CO2浓度升高的生理响应。在5个CO2浓度下(400,700,1 000,1 500和2 000 μatm)(1 atm=101×105 Pa)静止培养小球藻20代后,对其光合生理和生化组成进行分析。其中,升高CO2浓度增加了普通小球藻的细胞密度,且1 000 μatm是普通小球藻生长的最适浓度。实际光合效率与细胞密度呈现出相似的趋势。色素含量在1 000、1 500及2 000 μatm显著高于其他CO2浓度。CO2浓度升高会降低普通小球藻可溶性糖的含量,增加其蛋白质含量。除此之外,随着CO2浓度的升高,总酚物质的含量逐步后趋于平缓的趋势。油脂含量在1 500和2 000 μatm下显著高于其他CO2浓度下小球藻的油脂含量。当CO2浓度为700,1 000,1 500和2 000 μatm时,碳氮比与400 μatm时相比均发生了极显著的变化。不同CO2浓度下的不同生物组成的变化趋势有助于对未来环境下浮游植物对环境的响应做进一步研究。

关键词: 普通小球藻; CO2; 光合生理; 生化组成

 

中图分类号: R 931.711文献标志码: A

引用格式: 梁成伟, 王璐, 石蕾. 不同CO2浓度对普通小球藻生化组成及光合生理的影响\[J\]. 青岛科技大学学报(自然科学版), 2020, 41(3): 28-34.

 

LIANG Chengwei, WANG Lu, SHI Lei.Effects of different CO2 concentrations on the biochemical composition and photosynthetic physiology of Chlorella vulgaris\[J\]. Journal of Qingdao University of Science and Technology(Natural Science Edition), 2020, 41(3): 28-34.

 

Effects of Different CO2  Concentrations on the Biochemical Composition and

 Photosynthetic Physiology of Chlorella Vulgaris

 

LIANG Chengwei, WANG Lu, SHI Lei

(College of Marine Science and Biotechnology, Qingdao University of Science and Technology, Qingdao 266042,China)

 

Abstract: The physiological responses of Chlorella vulgaris to increased CO2 concentrations (400,700,1 000, 1 500 and 2 000 μatm) (1 atm=101×105 Pa) were investigated in this study. Photosynthetic physiology and biochemical composition of Chlorella vulgaris were measured after 20 generations of culture under elevated CO2 concentrations. The cell density increased significantly under elevated CO2 concentrations especially under 1 000 μatm. Actual photosynthesis efficiency and cell density showed similar trend which exhibited the most marked increase under 1 000 μatm. The difference between the pigments at the higher CO2 concentrations (1 000, 1 500 and 2 000 μatm) was little but much higher than other CO2 concentrations. Elevated CO2 has a significant inhibition on soluble carbohydrates. Within the limit of CO2 concentration, the increase of CO2 concentration will promote the protein content of Chlorella vulgaris. The lipid content and n(C)/n(N) under high CO2 concentrations changed significantly compared to 400 μatm. The trend of different biological compositions at different CO2 concentrations is benefit to further study of the response of phytoplankton to the environment in the future.

Key words: Chlorella vulgaris; CO2; photosynthetic physiology; biochemical composition

 

 

收稿日期:  2019-04-21

基金项目: 国家自然科学基金项目(31770393).

作者简介: 梁成伟(1979—),女,副教授.


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