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文章编号： 16726987（2019）05005507； DOI： 10.16351/j.16726987.2019.05.008
喻兆阳1,2， 薛慧颖1*
(1. 华中科技大学同济医学院附属同济医院 药学部，湖北 武汉 430030；2. 华中科技大学同济医学院 药学院，湖北 武汉 430030）
摘要： 制备一种可再生含钆介孔二氧化硅纳米粒（rMSNGd），对其进行表征，探究其应用于核磁共振成像（MRI）的可行性。以稻壳为硅源，掺杂金属钆，制备具有靶向显影功能的rMSNGd。透射电镜与扫描电镜观察该纳米材料特征，BET法与X射线衍射表征材料比表面积与介孔状态；能谱分析（EDS）及电感耦合等离子体发射光谱（ICPAES）检测纳米粒子元素含量；溶血实验表征其生物相容性；磁共振成像系统观察其在体外和BALB/c裸鼠体内Hela移植瘤的显像特征。制备的rMSNGd呈圆球形，分散性良好，表面具有规则的介孔结构，平均粒径为150 nm，钆含量（质量分数）为047%。材料具有良好生物相容性；体外MRI成像呈浓度梯度性；将该材料静脉注射裸鼠后约30 min，Hela裸鼠移植瘤信号强度明显增高。本研究成功制备了可再生含钆纳米介孔硅rMSNGd，其具肿瘤靶向聚集性，可作为新型磁共振造影剂使用。
关键词： 稻壳； 再生介孔二氧化硅纳米粒； 钆； 核磁共振成像
中图分类号： TB 321; R 445.2文献标志码： A
引用格式： 喻兆阳，薛慧颖. 可再生磁共振造影剂介孔二氧化硅纳米粒的制备与表征\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（5）： 5561.
YU Zhaoyang，XUE Huiying. Preparation and characterization of recycle MR imaging functional mesoporous silica nanoparticles\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(5)：5561.
Preparation and Characterization of Recycle MR Imaging
Functional Mesoporous Silica Nanoparticles
YU Zhaoyang1,2， XUE Huiying1
(1.Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030，
China; 2.School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030，China)
Abstract: A regenerative gadoliniumcontaining mesoporous silica nanoparticles (rMSNGd) were prepared and characterized, and its feasibility for magnetic resonance imaging (MRI) was explored.The rice husk is used as a silicon source and gadolinium was incorporated into a recycle mesoporous silica nanoparticle to prepare rMSNGd. The characteristics of the nanomaterials were observed by transmission electron microscopy and scanning electron microscopy.The specific surface area and mesoporous state of the materials were characterized by BET and Xray diffraction. The content of nanoparticles was detected by energy spectrum analysis (EDS) and inductively coupled plasma optical emission spectroscopy (ICPAES). The hemolysis experiment was used to evaluate its biocompatibility; the MRI system was used to observe the imaging characteristics in vitro and in vivo. The prepared rMSNGd was spherical and well dispersed. The globularshaped rMSNGd with Gadolinium content percentage of 47% was approximately 150 nm in diameter.These nanoparticles obtain excellent dispersibility and a regular mesoporous structure. The material has good biocompatibility; in vitro MRI imaging showed concentration gradient; about 30 min after intravenous injection of the material into nude mice, the signal intensity of Hela nude mice was significantly increased. A gadoliniumcontaining nanoparticle rMSNGd might be a novel nanoparticle contrast agent for magnetic resonance imaging.
Key words: rice husk； recycle； mesoporous silica nanoparticles； gadolinium； magnetic resonance imaging
收稿日期： 20181001
基金项目： 国家自然科学基金项目（81703446）；华中科技大学自主创新研究基金项目（514/5003514011）.
作者简介： 喻兆阳（1993—），男，药师.*通信联系人.
