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文章编号： 16726987（2019）05002506； DOI： 10.16351/j.16726987.2019.05.003
邱志伟， 接贵芬*
(青岛科技大学 化学与分子工程学院，山东 青岛 266042)
摘要： 通过使用二茂铁(Fc)功能化的还原氧化石墨烯(RGO)复合材料及核酸扩增技术构建了一个免标记且简易的电化学生物传感器，实现对食源性弧菌的高灵敏度地检测。探索了最佳的实验体条件，在最佳条件下，二茂铁的峰电流信号的改变与目标物的浓度有良好的线性关系，线性范围为10-22~10-15 mol·L-1，检测限为46×10-23 mol·L-1，该电化学生物传感器在生物分析和临床诊断领域具有潜在的应用价值。
关键词： 还原氧化石墨烯； 二茂铁； 核酸扩增； 食源性弧菌； 免标记； 生物传感器
中图分类号： O 063文献标志码： A
引用格式： 邱志伟， 接贵芬. 基于二茂铁功能化的石墨烯免标记的电化学生物传感器及核酸扩增技术实现对弧菌dsDNA的灵敏检测\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（5）： 2530.
QIU Zhiwei, JIE Guifen. Sensitive direct detection of dsDNA about vibrio using a labelfree electrochemical biosensor based on ferrocene functionalized reduced graphene oxide and nucleic acid amplification techniques\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(5)： 2530.
Sensitive Direct Detection of dsDNA about Vibrio Using a Labelfree
Electrochemical Biosensor Based on Ferrocene Functionalized Reduced
Graphene Oxide and Nucleic Acid Amplification Techniques
QIU Zhiwei, JIE Guifen
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: A labelfree and simple electrochemical biosensorwas constructedthat enables highly sensitive detection of Vibrio foodproducing bacteria by using ferrocene (Fc) functionalized reduced graphene oxide (RGO) composites and nucleic acid amplification techniques. As a good electron transfer medium, ferrocene is prepared by a noncovalent bond with redox graphene to form a composite material. Doublestranded (dsDNA) of Vibrio, the product of the amplification technique, combined with composite material by ππ electrostatic action. Due to it hinders the transfer of electrons, the response of the ferrocene as a signal molecule is greatly reduced, thus the target can be detected sensitively. Because DNA rarely exists in a singlestranded form in nature, most DNA and PCR amplification products are also doublestranded, so detecting dsDNA has important practical significance.In this experiment, the optimal experimental conditions were explored. Under the optimal conditions, the peak current signal of ferrocene has a good linear relationship with the concentration of the target. The linear range is 10-22—10-15 mol ·L-1. The limit is 46 × 10-23 mol ·L-1. The electrochemical biosensor has potential application value in the fields of biological analysis and clinical diagnosis.
Key words: reduced graphene oxide; ferrocene; nucleic acid amplification; vibrio; labelfree; electrochemical DNA biosensor
收稿日期： 20181012
基金项目： 国家自然科学基金项目(21575072).
作者简介： 邱志伟（1992—），女，硕士研究生.*通信联系人.
