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文章编号： 16726987（2019）04002507； DOI： 10.16351/j.16726987.2019.04.002
尹文硕， 张忠慧， 杨晓燕， 宋维玲*（青岛科技大学 化学与分子工程学院，山东 青岛 266042）
摘要： 利用银纳米粒子的金属荧光增强效应，构建荧光传感器实现对小分子ATP的检测。首先将修饰荧光基团的ATP适体固载在银纳米粒子表面，通过杂交互补将标记猝灭基团的DNA与之结合，实现荧光共振能量转移降低背景信号。ATP与适体链的特异性结合破坏荧光共振能量转移，荧光基团靠近银纳米粒子表面，由于银纳米粒子的金属荧光增强效应，使荧光信号增强，实现对ATP的检测，此种方法也可以用于其他生物小分子、蛋白质等的检测。
关键词： 金属荧光增强效应； 荧光共振能量转移； DNA； 信号放大
中图分类号： O 0657.3文献标志码： A
引用格式： 尹文硕， 张忠慧， 杨晓燕， 等. 基于金属荧光增强效应检测ATP的研究\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（4）： 2531.
YIN Wenshuo, ZHANG Zhonghui, YANG Xiaoyan, et al. Silver nanoparticlesenhanced fluorescence resonance energy transfer sensor for ATP detection\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(4)： 2531.
Silver NanoparticlesEnhanced Fluorescence Resonance Energy
Transfer Sensor for ATP Detection
YIN Wenshuo, ZHANG Zhonghui, YANG Xiaoyan, SONG Weiling
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: The silver nanoparticles (AgNPs)based fluorescence resonance energy transfer (FRET) was reported for ATP detection. The fluorophorefunctionalized ATP aptamer was first immobilized on the surface of the silver nanoparticles. Then, the quenchercarrying strands were hybridized with the aptamer to form FRET senor. When the senor interacted with ATP, the FRET effect was disrupted and the fluorescence intensity was enhanced by AgNPs. This method had good selectivity and had been successfully applied to the analysis of ATP in human serum samples. The method has potential application value in biological detection with wide application range, strong selectivity and convenient operation.
Key words: metalenhanced fluorescence effect; fluorescence resonance energy transfer; DNA; signal amplification
收稿日期： 20180808
基金项目： 国家自然科学基金项目(21705086).
作者简介： 尹文硕（1993—），男，硕士研究生.*通信联系人.
