响应面法优化山楂核总酚酸提取工艺及其降脂活性

PDF全文下载：  201904004.pdf

文章编号： 16726987（2019）04003808； DOI： 10.16351/j.16726987.2019.04.004

牛真真， 赵新玲， 郭遥遥， 公衍玲， 金宏*(青岛科技大学 化工学院，山东 青岛 266042)

摘要： 采用响应面法优化乙醇回流提取山楂核总酚酸的最佳工艺条件并研究其降脂活性。实验考察乙醇浓度、回流时间、液料比、提取温度与提取次数5个因素对山楂核总酚酸提取率的影响。在单因素试验的基础上，采用响应曲面法中的BoxBehnken模式，对山楂核总酚酸的提取工艺条件进一步优化，并研究山楂核提取物的胰脂肪酶抑制活性。结果发现，山楂核总酚酸的最佳提取工艺为乙醇浓度70%，回流时间25 h，液料比22 mL·g-1，提取温度86 ℃，提取次数2次，在此条件下山楂核总酚酸提取率为483 mg·g-1。山楂核提取物在体外有良好的胰脂肪酶抑制活性，具有剂量依赖性。响应面优化的提取工艺稳定可靠，可用于山楂核总酚酸的提取，获得的总酚酸提取物具有良好的降脂活性。

关键词： 山楂核； 总酚酸； 响应面优化； 胰脂肪酶

中图分类号： R 932文献标志码： A

引用格式： 牛真真， 赵新玲， 郭遥遥， 等. 响应面法优化山楂核总酚酸提取工艺及其降脂活性\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（4）： 3845.

NIU Zhenzhen, ZHAO Xinling, GUO Yaoyao,  et al. Optimization of extraction process of total phenolic acid from Crataegus pinnatifida seeds by response surface methodology and its lipidlower activity\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(4)： 3845.

Optimization of Extraction Process of Total Phenolic Acid from Crataegus Pinnatifida

Seeds by Response Surface Methodology and Its Lipidlower Activity

NIU Zhenzhen, ZHAO Xinling, GUO Yaoyao, GONG Yanling, JIN Hong

(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)

Abstract: The optimum conditions for total phenolic acid extraction from Crataegus pinnatifida seeds by ethanol reflux were optimized by response surface method and its lipidlowering activity was also studied. The effects of ethanol concentration, reflux time, liquidtoliquid ratio, extraction temperature and extraction times on the extraction rate of total phenolic acid from Crataegus pinnatifida seeds were investigated. Based on the single factor experiment, the BoxBehnken model in the response surface method was used to further optimize the extraction conditions of the total phenolic acid in the Crataegus pinnatifida seeds,and the inhibition on the pancreatic lipase activity of the Crataegus pinnatifida seeds extract was studied.The optimal extraction condition of total phenolic acid extraction from Crataegus pinnatifida seeds was ethanol concentration 70%, reflux time 25 h, liquidtoliquid ratio 22 mL·g-1, extraction temperature 86 ℃, extraction times 2 times.Under this condition, the total phenolic acid extraction rate of Crataegus pinnatifida seeds was 483 mg·g-1. Crataegus pinnatifida seeds extract has good pancreatic lipase inhibitory activity in a dosedependent manner. The response surface optimized extraction process is stable and reliable, and can be used for extraction of total phenolic acid from Crataegus pinnatifida nucleus.And the Crataegus pinnatifida seeds extract has good lipidlowering activity.

Key words: Crataegus pinnatifida seeds; total phenolic acid; response surface optimization; pancreatic lipase

收稿日期：  20181018

基金项目： 山东省重点研发计划项目（2018GSF119005）.

作者简介： 牛真真（1993—），女，硕士研究生.*通信联系人.