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文章编号： 16726987（2019）03003305； DOI： 10.16351/j.16726987.2019.03.006
苏曼卿， 郭遥遥， 赵新玲， 金宏， 公衍玲*
（青岛科技大学 化工学院，山东 青岛 266042）
摘要： 探讨延髓背侧迷走复合体（DVC）到下丘脑弓状核（ARC）ghrelin神经通路的构成并观察ghrelin受体（GHSR1a）在ARC的表达。采用逆行追踪结合荧光免疫组化染色的方法，观察DVC到ARC的ghrelin免疫阳性神经元的纤维投射；采用聚合酶链反应（Polymerase Chain Reaction，PCR）结合荧光免疫组化染色技术，观察GHSR1a在ARC的定性及定位表达。DVC发出ghrelin阳性神经元的纤维投射至ARC，并呈现出同侧投射的优势。ARC有GHSRla mRNA和蛋白的表达。DVC到ARC存在ghrelin神经通路，该通路的功能有待进一步研究。
关键词： ghrelin； 弓状核； 迷走复合体； 逆行追踪； 荧光免疫组化
中图分类号： R 965.2文献标志码： A
引用格式： 苏曼卿， 郭遥遥， 赵新玲， 等. 延髓背侧迷走复合体到下丘脑弓状核ghrelin神经通路的构成研究\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（3）： 3337.
SU Manqing, GUO Yaoyao, ZHAO Xinling, et al. Composition of the ghrelin neural pathway from the dorsal vagal complex to the arcuate nucleus of the hypothalamus\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(3)： 3337.
Composition of the Ghrelin Neural Pathway from the Dorsal Vagal Complex to the Arcuate Nucleus of the Hypothalamus
SU Manqing, GUO Yaoyao, ZHAO Xinling, JIN Hong, GONG Yanling
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: To investigate the composition of the ghrelin neural pathway from the Dorsal vagal complex (DVC) to the Arcuate nucleus (ARC) and observe the expression of growth hormone secretagogue receptor1a (GHSR1a) in ARC. Retrograde tracing andimmunofluorescence histochemical staining were used to observe the fiber projection of ghrelin immunopositive neuronfrom DVC to ARC. Polymerase chain reaction (PCR) andimmunofluorescence histochemical staining were used to observe the qualitative and localized expression of GHSR1a in ARC. DVC emitted fibers of ghrelinpositive neurons which projected to ARC and exhibited the advantage of ipsilateral projection. ARC had GHSRla mRNA and protein expression. There is a ghrelin neural pathway from DVC to ARC, and the function of this pathway needs further study.
Key words: ghrelin; arcuate nucleus; dorsalvagual complex; retrograde tracing; fluorescent immunohistochemistry
收稿日期： 20180713
基金项目： 山东省重点研发计划项目(2018GSF119005).
作者简介： 苏曼卿（1994—），女，硕士研究生.*通信联系人.
