PDF全文下载：
201903008.pdf
文章编号： 16726987（2019）03004309； DOI： 10.16351/j.16726987.2019.03.008
李凤梅1， 张洪亮1， 崔健2*， 刘素芹2， 宋云云2， 江志训2
(1.青岛科技大学 海洋科学与生物工程学院，山东 青岛 266042； 2.青岛市农业科学研究院，山东 青岛 266100）
摘要： 为培育耐低温强的南瓜砧木品种，本研究对‘青砧1号’、‘伟砧1号’和‘北瓜’进行耐低温性鉴定。在幼苗期对3个南瓜砧木品种进行常温（30 ℃/25 ℃）和低温（15 ℃/10 ℃）处理，测定其生理生化指标。低温胁迫下，3个南瓜砧木品种的可溶性糖含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性均明显升高，且所得抗寒性强弱结果与田间鉴定结果一致。可溶性糖含量、SOD、POD和CAT活性4个指标两两相关，可以用于评价南瓜砧木品种耐寒性的强弱。综合分析表明：‘青砧1号’抗寒性最好，‘伟砧1号’次之，‘北瓜’抗寒性最差。
关键词： 南瓜砧木； 低温胁迫； 生理生化指标
中图分类号： X 701文献标志码： A
引用格式： 李凤梅， 张洪亮， 崔健， 等. 低温胁迫对砧用南瓜生理生化指标的影响\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（3）： 4351.
LI Fengmei， ZHANG Hongliang， CUI Jian, et al. Effect of low temperature stress on physiological and biochemical indexes of pumpkin rootstock seedlings\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(3)： 4351.
Effect of Low Temperature Stress on Physiological and Biochemical Indexes of Pumpkin Rootstock Seedlings
LI Fengmei1， ZHANG Hongliang1， CUI Jian2， LIU Suqin2， SONG Yunyun2， JIANG Zhixun2
(1.College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; 2.Qingdao Institute of Agricultural Science Research, Qingdao 266100, China)
Abstract: In order to breed pumpkin rootstock with low temperature tolerance, this study evaluated the low temperature tolerance of "Qingzhen No.1", "Weizhen No.1" and "Beigua". The seedlings of three pumpkin rootstocks were treated with normal temperature (30 ℃/25 ℃) and low temperature (15 ℃/10 ℃), the physiological and biochemical indexes of different pumpkin stocks were studied. Soluble sugar content, superoxide dismutase (SOD), peroxydase (POD), and catalase ( CAT ) activities significantly increased, the obtained results were consistent with field identification results of cold tolerance. Soluble sugar content, SOD, POD and CAT activities were related to each other, which can be used as the suitable indexes to evaluate the cold tolerance of pumpkin rootstocks. Comprehensive analysis indicated that pumpkin rootstocks "Qingzhen No. 1" was appeared best in cold tolerance, followed by "Weizhen No.1", and "Beigua" has the worst cold tolerance. This study provide a theoretical basis for breeding of pumpkin rootstocks with low temperature tolerance.
Key words: pumpkin rootstocks； low temperature stress； physiological and biochemical properties
收稿日期： 20180425
基金项目： 山东省现代农业产业技术体系项目（SDAIT0502）；青岛市民生科技计划项目（1861116nsh）.
作者简介： 李凤梅（1974—），女，副教授.*通信联系人.
