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文章编号： 16726987（2019）02003006； DOI： 10.16351/j.16726987.2019.02.004
孙晓娟， 许泳吉*
(青岛科技大学 化学与分子工程学院，山东 青岛 266042)
摘要： 采用高效液相色谱紫外检测法(HPLCUV)同时测定了有色饮料中的合成色素柠檬黄(E102)、日落黄(E110)和诱惑红(E129)。样品经溶解、超声去除气泡、稀释、045 μm的滤膜过滤后，直接用HPLC分析。考察了流动相的体系及比例、紫外测定波长和柱温对3种色素的分离检测效果的影响。确定流动相为V(甲醇)∶V(乙酸铵(002 mol·L-1))=40∶60，紫外检测波长为254 nm，柱温为30 ℃。结果表明：柠檬黄、日落黄和诱惑红在该条件下均有较好的分离和检测，定量范围分别为030~10、050~10和10~10 μg·mL-1，3种色素的线性相关系数R2为0999 0~0999 3，检出限为009~050 μg·mL-1，相对标准偏差为007%~016%，回收率为9477%~10023%。该方法样品预处理简单、分离效果好，适用于饮料中柠檬黄、日落黄和诱惑红的同时测定。
关键词： 高效液相色谱； 饮料； 柠檬黄； 日落黄； 诱惑红
中图分类号： O 657.7文献标志码： A
引用格式：孙晓娟， 许泳吉. 高效液相色谱法同时测定饮料中柠檬黄、日落黄和诱惑红的含量\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（2）： 3035.
SUN Xiaojuan, XU Yongji. Simultaneous determination of tartrazine, sunset yellow and allura red in beverages by high performance liquid chromatography\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(2)： 3035.
Simultaneous Determination of Tartrazine, Sunset Yellow and Allura Red in
Beverages by High Performance Liquid Chromatography
SUN Xiaojuan, XU Yongji
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology，Qingdao 266042, China)
Abstract: In this paper, tartrazine (E102), sunset yellow (E110), and allura red (E129) were determined by high performance liquid chromatography with UV detector (HPLCUV). The samples were dissolved and degassed by ultrasonication, diluted with water, filtered through 045 μm millipore filters, and analyzed by HPLC. The effects of mobile phase, UV detection wavelength, and temperature on the separation of three pigments were investigated. It was finally determined that the mobile phase was V(methanol)∶V(ammonium acetate (002 mol·L-1))= 40∶60, the detection wavelength was 254 nm, and the temperature was 30 ℃. The results showed that tartrazine, sunset yellow, and allura red were well separated and detected under the above conditions.The limits of quantification of three pigments were 030—10, 050—10 and 10—10 μg·mL-1, respectively. Correlation coefficients (0999 0—0999 3), limits of detection (009—050 μg·mL-1), standard deviations of reproducibility (007%—016%) and recoveries (9477%—10023%) were determined. The method with the properties of simple pretreatment and good effect is suitable for simultaneous quantitative the content of synthetic pigments in beverages.
Key words:high performance liquid chromatography； beverages； tartrazine； sunset yellow； allura red
收稿日期： 20180408
基金项目： 山东省重点研发计划项目（2018GGX104005）.
作者简介： 孙晓娟（1993—），女，硕士研究生.*通信联系人.
