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文章编号： 16726987（2018）06002105； DOI： 10.16351/j.16726987.2018.06.003
陈凤廷， 张晓茹， 牛淑妍*
（青岛科技大学 化学与分子工程学院，山东 青岛 266042）
摘要： 通过利用DNA合成的银纳米簇作为荧光探针研究一种新型的检测汞离子的方法。该方法是基于汞离子触发使颈环DNA的颈部形成双链结构，核酸外切酶Ⅲ剪切双链部分释放出DNA单链，该DNA单链能作为模板用于形成荧光银纳米簇，该传感器的荧光信号与汞离子的浓度有关。释放的汞离子可以继续参与循环，进一步放大检测信号。汞离子检测的线性范围是30×10-11到30×10-8 mol·L-1，该传感器的灵敏度较高。该方法是对汞离子的特异性检测，不受其他离子的影响，并可用于实际样品的检测。
关键词： 银纳米簇； 汞离子； 核酸外切酶Ⅲ
中图分类号： O 657.3文献标志码： A
引用格式： 陈凤廷， 张晓茹， 牛淑妍. 利用纳米银簇循环放大检测汞离子\[J\]. 青岛科技大学学报（自然科学版）， 2018， 39（6）： 2125.
CHEN Fengting, ZHANG Xiaoru, NIU Shuyan. Detection of mercury ion by cyclic amplification of silver clusters\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2018, 39(6)： 2125.
Detection of Mercury Ion by Cyclic Amplification of Silver Clusters
CHEN Fengting, ZHANG Xiaoru, NIU Shuyan
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: A novel method for detecting Hg2+ ion was developed using silver nanoclusters as fluorescent probe. The stemloop structure DNA could form a closed double stranded structure in presence of Hg2 + ions. Then Exo Ⅲ could recognize and shear the double stranded portion to release DNA single strand. The released DNA single strand acted as a template for the preparation of fluorescent AgNCs. Thus the fluorescence of the AgNCs could be used as a signal transduction of this sensor, which was related to the concentration of the Hg2+ ions. The released target Hg2 + ions could initiate another cycle of the cleavage reaction and leading to signal ampliflcation in the same way. A linear response to the concentration of Hg2+ ions was observed in the range from 30 × 10-11 to 30 × 10-8 mol·L-1. This sensor was specific for Hg2+ ions without being affected by other metal ions. At the same time, it might find important applications for actual sample.
Key words: silver nanoclusters; mercury ion; Exo Ⅲ
收稿日期： 20170717
基金项目： 国家自然科学基金项目（21275003）.
作者简介： 陈凤廷(1991—)，女，硕士研究生.*通信联系人.
