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文章编号： 16726987（2018）05002106； DOI： 10.16351/j.16726987.2018.05.003
华晓雨, 孙桐, 玄光善*
(青岛科技大学 化工学院，山东 青岛 266042)
摘要： 采用溶剂挥发法制备地塞米松温敏水凝胶。考察紫外灭菌、臭氧灭菌及电子束辐照灭菌对地塞米松温敏水凝胶的灭菌效果，并对灭菌后地塞米松温敏水凝胶的细胞毒性进行了评价。结果显示，电子束辐照灭菌后水凝胶中的细菌和真菌数为0，说明电子束辐照灭菌对地塞米松温敏水凝胶灭菌效果良好。高、中、低剂量的供试品和无药组凝胶的相对增值率（RGR）分别为968%、870%、816%及812%，说明高、中、低剂量和无药组的地塞米松温敏水凝胶细胞毒性较小，其细胞毒性符合相关要求。
关键词： 地塞米松； 温敏水凝胶； 灭菌； 细胞毒性
中图分类号： R 944文献标志码： A
引用格式： 华晓雨, 孙桐, 玄光善. 地塞米松温敏水凝胶的制备及其灭菌方式和细胞毒性研究\[J\]. 青岛科技大学学报（自然科学版）， 2018， 39（5）： 2126.
HUA Xiaoyu， SUN Tong， XUAN Guangshan. Preparation, sterilization method and cytotoxicity study of dexamethasone temperaturesensitive hydrogel\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2018, 39(5)： 2126.
Preparation, Sterilization Method and Cytotoxicity Study of
Dexamethasone Temperaturesensitive Hydrogel
HUA Xiaoyu， SUN Tong， XUAN Guangshan
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: Dexamethasone temperaturesensitive hydrogel was prepared by the solvent evaporation method. The sterilization effects of ultraviolet, ozone and electron beam irradiation sterilization methods for gel were studied, respectively. And the cytotoxicity of the gel was evaluated after sterilization. The results showed that both the numbers of bacteria and fungi in hydrogel were zero after sterilized by the method of electron beam irradiation, which indicated that electron beam irradiation sterilization was an effective sterilization method for dexamethasone temperaturesensitive hydrogel. The relative growth rate of the high, medium, low dose and no drug groups were 968%, 870%, 816% and 812% respectively, which indicated that the cytotoxicity of dexamethasone temperaturesensitive hydrogel was low, and the cytotoxicity of the gel meets the requirements.
Key words: dexamethasone; temperaturesensitive hydrogel; sterilization; cytotoxicity
收稿日期： 20170904
基金项目： 国家自然科学基金项目（31760521）.
作者简介： 华晓雨（1994—），女，硕士研究生.*通信联系人.
