PDF全文下载：2017050040
刘洋1， 钟逸凡2， 苏曼卿1， 刘菲1， 公衍玲1*
(1.青岛科技大学 化工学院，山东 青岛 266042； 2.青岛市第二中学， 山东 青岛 266000)
摘要： 研究顺铂对大鼠血浆、胃及孤束核（NTS）ghrelin及其受体表达的影响。将大鼠分为顺铂组和生理盐水组。记录摄食、高岭土摄入量和体重。48 h后分别取血分离血浆，采用Elisa法测定ghrelin浓度；取胃组织冷冻保存，采用PCR法测定ghrelin及其受体GHS-R1a mRNA的表达；取NTS冷冻保存，采用Western blot和PCR法测定ghrelin和ghrelin受体GHS-R1a蛋白及其mRNA表达水平。顺铂组与生理盐水组比较，食物摄入量减少（p<0 .05）；高岭土消耗量显著增加（p<0.01）；体重减少但无统计学意义（p>0.05）；血浆ghrelin浓度降低但无统计学意义（p>0.05）；胃ghrelin mRNA表达下降，但无显著意义（p>0.05），受体GHS-R1a mRNA表达显著增加（p<0 .05）；nts ghrelin蛋白及其mrna表达下降（p<0.05），受体ghs-r1a蛋白及其mrna表达显著增加（p<0.05）。ghrelin可能参与顺铂引起的恶心呕吐。
关键词： 顺铂； 摄食； 体重； ghrelin； 高岭土
中图分类号： R 363文献标志码： A
引用格式：刘洋， 钟逸凡， 苏曼卿， 等. 顺铂处理大鼠ghrelin的变化及意义\[J\]. 青岛科技大学学报（自然科学版）， 2017， 38（5）： 40-43.
LIU Yang, ZHONG Yifan, SU Manqing, et al. Changes and significance of ghrelin in rats treated with cisplatin\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2017, 38(5)： 40-43.
Changes and Significance of Ghrelin in Rats Treated with Cisplatin
LIU Yang1, ZHONG Yifan2, SU Manqing1, LIU Fei1, GONG Yanling1
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
2.Qingdao No.2 High School, Qingdao 266000)
Abstract: To study the effect of cisplatin on the expression of ghrelin and its receptors in plasma, stomach and nucleus of solitary tract (NTS) in rats. The rats were divided into the cisplatin group and the saline group. The feeding, kaolin intake and body weight were recorded. After 48 h, blood plasma was separated, and ghrelin concentration was determined by Elisa method; Cryopreservation of gastric tissue, the expression of ghrelin and its receptor mRNA GHS-R1a was determined by PCR method. Cryopreservation of NTS, ghrelin and ghrelin receptor GHS-R1a protein and mRNA expression levels were determined by Western blot and PCR methods respectively. Compared with saline group, the food intake in cisplatin group decreased (p<0 .05); kaolin consumption was increased significantly (p<0.01); body="" weight="" decreased="" with="" no="" statistical="" significance="" p="">0.05); plasma ghrelin concentration decreased but no statistical significance (p>0.05); gastric mRNA expression of ghrelin decreased, but there was no significant meaning (p>0.05), while mRNA expression of ghrelin receptor GHS-R1a significantly increased (p<0 .05); mrna expression of ghrelin in nts decreased as well as its protein expression (p<0.05), while ghrelin receptor ghs-r1a expression significantly increased (p<0.05). ghrelin may be involved in cisplatin induced nausea and vomiting.
Key words: cisplatin; feeding; body weight; ghrelin; kaolin
收稿日期： 2016-07-17
基金项目： 国家自然科学基金青年基金项目(81300281);山东省高校科技计划项目(J15LK12).
作者简介： 刘洋（1990—），女，硕士研究生.*通信联系人.
文章编号： 1672-6987（2017）05-0040-04； DOI： 10.16351/j.1672-6987.2017.05.007
