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电子束辐照与邻酪氨酸产率的剂量关系

作者:时间:2017-10-24点击数:

PDF全文下载:2017050036

张丹丹, 王欣欣

(青岛科技大学 化工学院,山东 青岛 266042)

摘要: 阐明了辐照剂量与邻酪氨酸转化率的关系。以邻酪氨酸的前体苯丙氨酸为研究对象,给予不同电子束辐照剂量,使用液相色谱-串联质谱联用法同时测定3种酪氨酸同分异构体和苯丙氨酸,探讨了在无基质干扰条件下辐照剂量与邻酪氨酸转化率的关系,以及苯丙氨酸在不同辐射剂量下转化为邻酪氨酸的效率。结果表明:在辐照剂量为1~10 kGy时,邻酪氨酸的转化率为0.019%~0.105%,远高于样品基质中邻酪氨酸的转化率。

关键词: 辐照剂量; 邻酪氨酸; 苯丙氨酸; 转化; 液相色谱-串联质谱联用法

中图分类号:  O 657.6; O 657.7文献标志码: A

引用格式:张丹丹, 王欣欣. 电子束辐照与邻酪氨酸产率的剂量关系\[J\]. 青岛科技大学学报(自然科学版), 2017, 38(5): 36-39.

ZHANG Dandan, WANG Xinxin. Relationship between o-tyrosine content and irradiation dose in phenylalanine with the electron beam irradiation\[J\]. Journal of Qingdao University of Science and Technology(Natural Science Edition), 2017, 38(5): 36-39.

Relationship Between o-Tyrosine Content and Irradiation Dose in Phenylalanine

with the Electron Beam Irradiation

ZHANG Dandan, WANG Xinxin

(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)

Abstract: In this study, the relationship between the contents of o-tyrosine in phenylalanine and the electron beam irradiation dose was studied. Phenylalanine, as the precursor of o-tyrosine, was irradiated by electron beam with different irradiated dose, then a stable LC-MS/MS method was developed for the simultaneous determination of three tyrosine isomers and phenylalanine, the relationship between the irradiation dose and o-tyrosine content was investigated without the interference of matrix, and the efficiency of phenylalanine conversion to o-tyrosine was analyzed under different irradiation doses. The results showed that the conversion of phenylalanine to o-tyrosine at different irradiated dose was of 0.019%—0.105% at 1—10 kGy dose in a dose-dependent manner, much more higher than that in seafood which indicated that the matrix of seafood would produce effect on the conversion of phenylalanine to o-tyrosine.

Key words: irradiation dose; o-tyrosine; phenylalanine; conversion; liquid chromatography-tandem mass spectrometric

收稿日期: 2016-06-06

基金项目: 山东省高等学校科研计划项目(J15LM62).

作者简介: 张丹丹(1978—),女,副教授.

文章编号: 1672-6987(2017)05-0036-04; DOI: 10.16351/j.1672-6987.2017.05.006

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