PDF全文下载：2017050036
张丹丹， 王欣欣
（青岛科技大学 化工学院，山东 青岛 266042）
摘要： 阐明了辐照剂量与邻酪氨酸转化率的关系。以邻酪氨酸的前体苯丙氨酸为研究对象，给予不同电子束辐照剂量，使用液相色谱-串联质谱联用法同时测定3种酪氨酸同分异构体和苯丙氨酸，探讨了在无基质干扰条件下辐照剂量与邻酪氨酸转化率的关系，以及苯丙氨酸在不同辐射剂量下转化为邻酪氨酸的效率。结果表明：在辐照剂量为1~10 kGy时，邻酪氨酸的转化率为0.019%~0.105%，远高于样品基质中邻酪氨酸的转化率。
关键词： 辐照剂量； 邻酪氨酸； 苯丙氨酸； 转化； 液相色谱-串联质谱联用法
中图分类号： O 657.6； O 657.7文献标志码： A
引用格式：张丹丹， 王欣欣. 电子束辐照与邻酪氨酸产率的剂量关系\[J\]. 青岛科技大学学报（自然科学版）， 2017， 38（5）： 36-39.
ZHANG Dandan, WANG Xinxin. Relationship between o-tyrosine content and irradiation dose in phenylalanine with the electron beam irradiation\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2017, 38(5)： 36-39.
Relationship Between o-Tyrosine Content and Irradiation Dose in Phenylalanine
with the Electron Beam Irradiation
ZHANG Dandan, WANG Xinxin
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: In this study, the relationship between the contents of o-tyrosine in phenylalanine and the electron beam irradiation dose was studied. Phenylalanine, as the precursor of o-tyrosine, was irradiated by electron beam with different irradiated dose, then a stable LC-MS/MS method was developed for the simultaneous determination of three tyrosine isomers and phenylalanine, the relationship between the irradiation dose and o-tyrosine content was investigated without the interference of matrix, and the efficiency of phenylalanine conversion to o-tyrosine was analyzed under different irradiation doses. The results showed that the conversion of phenylalanine to o-tyrosine at different irradiated dose was of 0.019%—0.105% at 1—10 kGy dose in a dose-dependent manner, much more higher than that in seafood which indicated that the matrix of seafood would produce effect on the conversion of phenylalanine to o-tyrosine.
Key words: irradiation dose; o-tyrosine; phenylalanine; conversion; liquid chromatography-tandem mass spectrometric
收稿日期： 2016-06-06
基金项目： 山东省高等学校科研计划项目(J15LM62).
作者简介： 张丹丹（1978—），女，副教授.
文章编号： 1672-6987（2017）05-0036-04； DOI： 10.16351/j.1672-6987.2017.05.006
