PDF全文下载：2017030038
公衍玲，钟逸凡，苏曼卿，刘菲
(青岛科技大学 化工学院,山东 青岛266042)
摘要： 通过研究猪毛菜（Salsola collina）对小鼠肠运动的影响，深入探讨其作用机制。建立正常小鼠肠推进模型，检测大、中、小剂量（0.025，0.05，0.1 g·mL-1）猪毛菜对肠推进的影响。分别采用阿托品和肾上腺素建立小鼠肠推进减慢模型，观察不同剂量猪毛菜对肠推进的影响。通过酶联免疫吸附试验(Elisa法)分别测定猪毛菜对正常小鼠血清和肠组织中的胃动素和胃饥饿素含量的影响。实验结果表明：大、中、小剂量猪毛菜对正常小鼠肠运动具有显著促进作用（分别为p<0 .05，p<0.01和p<0.01），还可显著改善阿托品致小鼠肠推进减慢作用（p<0.01），但对肾上腺素致小鼠肠推进减慢无显著影响（p>0.05）。猪毛菜能显著提高血清中胃饥饿素含量（p<0 p="">0.05）。猪毛菜可通过激动M-胆碱能受体促进胃饥饿素合成，多靶点促进胃肠运动。
关键词：猪毛菜；阿托品；肾上腺素；促肠运动
中图分类号：R 932文献标志码：A
引用格式：公衍玲，钟逸凡，苏曼卿，等.猪毛菜促进小鼠小肠运动的机理\[J\].青岛科技大学学报（自然科学版），2017，38（2）：38-41.
GONG Yanling, ZHONG Yifan, SU Manqing， et al.Mechanism of Salsola collina on promoting small intesinal peristalsis in mice \[J\].Journal of Qingdao University of Science and Technology（Natural Science Edition),2017,38(2)：38-41.
Mechanism of Salsola Collina on Promoting Small Intesinal Peristalsis in Mice
GONG Yanling, ZHONG Yifan, SU Manqing, LIU Fei
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)Abstract: The effect and its mechanism of Salsola collina on intestinal motility in mice was explored. Normal mouse intestinal propulsion model was established and the influence of different dosage (0.025,0.05,0.1 g·mL-1)of Salsola collina on intestinal propulsion was detected. Prolonged intestinal propulsion of mice model induced by atropine and adrenaline was established to observe the influence of different dosage of Salsola collina on intestinal propulsion.The effects of Salsola collina on serum and intestinal motilin and ghrelin in normal mouse were detected by enzyme linked immunosorbent assay (Elisa). The results showed that three doses of Salsola collina significantly promoted intestinal motility in normal mice (p<0 .05, p<0.01 and p<0.01), and significantly improved the atropine induced intestinal propusion(p<0.01), while="" had="" no="" effect="" on="" adrenaline="" induced="" intestinal="" propulsion="" p="">0.05). Salsola collina can significantly improved the content of serum ghrelin (p<0 but="" had="" no="" effect="" on="" serum="" and="" intestinal="" motilin="" ghrelin="" p="">0.05). Salsola collina is possibly promotes intestinal motility by multiple targets such as exciting M-cholinergic receptor and promote ghrelin synthesis.
Key words: Salsola collina; atropine; adrenaline; promote intestinal motility
收稿日期：2016-01-20
基金项目：国家自然科学基金青年基金项目(81300281);山东省高校科技计划项目(J15LK12).
作者简介：公衍玲（1975—），女，副教授.
文章编号：16726987（2017）03003804； DOI： 10.16351/j.1672-6987.2017.03.007
