PDF全文下载：2017020027
王菲，付甜，许泳吉*
（青岛科技大学化学与分子工程学院，山东青岛266042）
摘要：利用固相萃取-高效液相色谱法同时测定饮水纸杯中荧光增白剂2,5-双-(5-叔丁基-2-苯并恶唑基)噻吩（OB）和4,4-双(2-甲氧基苯乙烯基)联苯（CBS-127）的含量，样品经二氯甲烷超声提取，CN固相萃取柱纯化后，以V(乙腈)∶V(甲醇)∶V(水)（20∶75∶5）为流动相，高效液相色谱-荧光检测器测定。结果表明：荧光增白剂OB和CBS-127可以得到较好的分离检测，混合溶液在0.01~1 mg·L-1范围内线性关系良好，OB的线性关系为Y=30.938X+1.396 8，相关系数为0.999 6，检出限为1.5×10-3 mg·kg-1，相对标准偏差为1.24%；CBS-127的线性关系为Y=62.271X+1.589 8，相关系数为0.999 8，检出限为3×10-3 mg·kg-1，相对标准偏差为1.33%。实际样品的加标平均回收率为79%~92%，该方法样品处理简单、精密度好，适用于同时测定饮水纸杯中OB和CBS-127。
关键词：高效液相色谱；纸杯；固相萃取；OB；CBS-127
中图分类号：TQ 610.495文献标志码：A
引用格式：王菲，付甜，许泳吉.固相萃取-高效液相色谱法同时测定饮水纸杯中荧光增白剂OB和CBS-127的含量\[J\].青岛科技大学学报（自然科学版），2017，38（2）：27-33.
WANG Fei, FU Tian, XU Yongji.Simultaneous determination of drinking cups of fluorescent whitening agents OB and CBS-127 by solid phase extraction and high performance liquid chromatography\[J\].Journal of Qingdao University of Science and Technology（Natural Science Edition),2017,38(2)：27-33.
Simultaneous Determination of Drinking Cups of Fluorescent Whitening Agents OB and CBS-127 by Solid Phase Extraction and High Performance Liquid Chromatography
WANG Fei, FU Tian, XU Yongji
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract:Simultaneous determination the content of the paper drinking water cup of fluorescent whitening agent 2,5-di(5-tert-butylbenzoxazol-2-yl) thiophene (OB) and 4,4′-bis(2-methoxystyryl)-1,1′-biphenyl (CBS-127) with solid phase extraction method using high performance liquid chromatography（HPLC）, the samples were ultrasound extracted by dichloromethane, purified by CN solid phase extraction, V (acetonitrile）∶V（methanol）∶V（water）=20∶75∶5 as mobile phase, HPLC fluorescence detector determination，the resultsshows that: the fluorescent whitening agent OB and CBS-127 can be isolated well, the mixed solution at range of 0.01—1 mg·L-1 have a good linear relationship, the fluorescent whitening agent OB was Y=30.938X+1.396 8, the correlation coefficient was 0.999 6, the detection limit is 1.5×10-3 mg·kg-1, the relative standard deviation was 1.24%, the linear relationship of the fluorescent whitening agent CBS-127 was Y=62.271X+1.589 8, the correlation coefficient was 0.999 8, the detection limit is 3×10-3 mg·kg-1, the relative standard deviation is 1.33%. average spiked recovery rate was 79%—92%. The sample treatment is simple, precision, it is suitable for simultaneous determination of drinking cups of fluorescent whitening agents in OB and CBS-127.
Key words: high performance liquid chromatography; paper cup; solid phase extraction; OB; CBS-127
收稿日期：2015-11-13
作者简介：王菲（1991—），女，硕士研究生.*通信联系人.
文章编号：1672-6987（2017）02-0027-07；DOI：10.16351/j.1672-6987.2017.02.003
