响应面法优化丹参叶总酚酸的提取工艺及其对酪氨酸酶的抑制活性

PDF全文下载：20160100013

1.青岛科技大学 化工学院，山东 青岛 266042；2.瑞康中药饮片有限公司，山东 平邑 273300)

 摘要： 采用热水浸提法提取丹参叶中总酚酸，优化提取工艺并检测提取物对酪氨酸酶的抑制作用。以总酚酸得率为指标，采用星点设计响应面法考察液料比、提取温度和提取时间对提取工艺的影响，采用多元线性回归和二项式方程拟合，确定最佳提取工艺。以左旋多吧为底物，观察丹参叶总酚酸提取物对酪氨酸酶的抑制作用。实验结果表明：丹参叶总酚酸的最佳工艺条件为液料比23∶1，提取时间109 min，提取温度76 ℃。该条件下总酚酸得率预测值为3.211%，实测平均值为3.285%。丹参叶总酚酸提取物可抑制酪氨酸酶的活性，并具有一定的剂量依赖关系。星点设计响应面法可用于丹参叶总酚酸的提取工艺优化，预测性良好；获得的提取物具有一定的美白效果。

关键词： 丹参叶；总酚酸；提取；响应面法；酪氨酸酶

 中图分类号： R 284.2              文献标志码： A

  Optimization of Extraction of Total Phenolic Acids from Leaves of Radix Salvia

Miltiorrhiza Using Response Surface Methodology and Its Inhibition on Tyrosinase

  GONG Yanling1， QIU Zhiqi1, LIU Fei1, YAN Guangfu2

 (1.College of Chemical Engineering， Qingdao University of Science and Technology, Qingdao 266042，China;

2.Ruikang Herbal Pieces Co., Ltd.,  Pingyi 273300，China)

 Abstract: Total phenolic acids from leaves of Radix salvia Miltiorrhiza were extracted by hot water extraction, and the optimal conditions for extraction were determined and its inhibition on tyrosinase was observed. The effects of liquidtosolid ratio, extraction temperature and extraction time on the total phenolic acids yield were observed using central composite designresponse surface methodology (CCDRSM). The extraction condition was optimized by multiple linear regression and binomial equation. The inhibition on tyrosinase of total phenolic acids extracts was measured by the utilization of LDA as substrate. The results revealed that the optimal condition for total phenolic acids from leaves of Radix salvia Miltiorrhiza were as follows: Liquidtosolid ratio 23∶1, extraction time 109 min and extraction temperature 76 ℃. Under these conditions, the predicted and experimental extraction yields of total phenolic acids were 3211% and 3285% respectively. The total phenolic acids extracts inhibited the tyrosinase activity with a certain dosedependent manner. CCDRSM could be used for optimization of extraction technology of total phenolic acids from leaves of Radix salvia Miltiorrhiza with good predictability and the extracts obtained shows a whitening effect.

 Key words: leaves of Radix salvia Miltiorrhiza; total phenolic acids; extraction; response surface methodology; tyrosinase

公衍玲1， 邱治淇1， 刘菲1， 闫广福2

 (收稿日期： 20141107

作者简介： 公衍玲（1975—），女，副教授.

文章编号： 16726987（2016）01001305； DOI： 10.16351/j.16726987.2016.01.003