固相萃取高效液相色谱法同时测定烟草中吡虫啉和多菌灵

PDF全文下载：20160100026

许泳吉，刘云云，韩玮，王菲

（青岛科技大学 化学与分子工程学院，山东 青岛 266042）

 摘要：建立了一种同时测定烟草中吡虫啉和多菌灵农药残留的高效液相色谱方法。样品经丙酮超声提取，Cleanert CN固相萃取柱净化、富集。采用甲醇002 mol·L-1磷酸盐缓冲溶液（pH=74）为流动相，配备ODSC18柱、紫外检测器的高效液相色谱（HPLC）仪对待测组分进行了分离和测定。结果表明：吡虫啉和多菌灵在02~20 μg·mL-1的范围内线性良好，相关系数分别为0999 9和0997 3。多菌灵和吡虫啉的添加回收率分别在873%~957%和847%~945%之间，相对标准偏差（RSD）均小于35%。

 关键词： 高效液相色谱法；吡虫啉；多菌灵；烟草

 中图分类号： O 657.7+2文献标志码：A

 Simultaneous Detection of Carbendazim and Imidacloprid in Tobacco with Solidphase Extraction and Highperformance Liquid Chromatography

XU Yongji, LIU Yunyun, HAN Wei, WANG Fei

(College of Chemistry and Molecular Engineering, Qingdao University of Sicence and Technology, Qingdao 266042, China)

Abstract: A analytical method of carbendazim and imidacloprid pesticide residues in tobacco by high performance liquid chromatography (HPLC) was developed. Samples was extracted with acetone and cleaned up through the Cleanert CN SPE. The HPLC conditions were ODSC18 colum, methanol0.02 mol·L-1 phosphate buffered saline (pH=7.4) as mobile phase, and UV detection at 278 nm. The results showed that carbendazim and imidacloprid in the concentration range of 02—20 μg·mL-1 have a good linear relationship, the correlation coefficient are 0.997 3 and 0999 9 respectively. The fortified recoveries of carbendazim and imidaclorpid in tobacco samples were 873%—957% and 847%—945%, respectively. Relative standard deviation was both less than 35%.

 Key words: high performance liquid chromatography; carbendazim; imidacloprid; tobacco

 收稿日期： 20141221

作者简介： 许泳吉(1960—)，男，教授.

文章编号： 16726987（2016）01002606； DOI： 10.16351/j.16726987.2016.01.006