基于纳米钴信号放大化学发光生物传感器超灵敏检测DNA

曹玮， 张远

(青岛科技大学 化学与分子工程学院，山东 青岛 266042)

 摘要： 研究了一种新颖简便的以大粒径（100 nm）Co纳米探针作为信号放大超灵敏化学发光检测DNA的新方法。该方法采用“三明治”夹心法，首先将壳核结构羧基化的纳米Co与氨基修饰的DNA结合制备纳米Co信号探针，然后通过靶DNA 与固定了捕获探针的磁珠亲和形成一个“三明治”夹心的结构，制备纳米Co生物传感器。检测时，将纳米Co溶解成Co2+，利用鲁米诺H2O2Co2+化学发光体系对靶DNA进行检测，发现其对靶DNA的检测灵敏度可达到16×10-17 mol·L-1（3δ），其线性范围为06×10-16 ~ 40×10-16 mol·L-1。

关键词： 纳米Co； 化学发光；  DNA检测

中图分类号： O 65                  文献标志码： A

Chemiluminescence Detection of DNA Hybridization Based on Cobalt Nanoparticles

 CAO Wei, ZHANG Yuan

(College of Chemistry and Molecular Engineering，Qingdao University of Science and Technology, Qingdao 266042，China)

Abstract： A novel and sensitive flow injection chemiluminescence(CL) assay for the sequence specific DNA detection based on the signal amplication with cobalt nanoparticles(100 nm) was developed. The sandwichtype DNA biosensor was fabricated with the aminofunctionalized capture DNA immobilized on the magnetic bead and target DNA was sandwiched between magnetic bead and a signal DNA probe labeled with cobalt NPs. The hybridization events were monitored by the Chemiluminescence (CL) intensity of luminolH2O2CO2+ after the cobaltous ions were dissolved from the hybrids. The method could detect as low as 16 × 10-17 mol·L-1 target DNA and the line range was from 06 × 10-16 to 40 × 10-16 mol·L-1.

Key words: cobalt nanoparticles; chemiluminescence (CL); DNA detection

收稿日期： 20140410

基金项目： 山东省自然科学基金项目（ZR2012BM011）；青岛科技大学博士启动基金项目（2011）.

作者简介： 曹玮（1968—）,女，高级实验师.