全文下载：2013060579
刘路宽， 苏忠亮， 李露， 于世涛*
(青岛科技大学 化工学院，山东 青岛 266042)
摘要： 采用共缩聚法制备了氨基功能化SBA-15介孔分子筛，以此为载体，戊二醛作交联剂，进行了纤维素酶固定化。考察了戊二醛浓度、给酶量、pH、固定化时间等因素对固定化的影响，确定了固定化最佳条件，并对固定化酶的酶学性质进行了研究。结果表明，固定化酶最适作用温度60 ℃，最适作用pH为4；固定化酶Km值3.61 mg·mL-1；固定化酶的热稳定性、操作稳定性、贮存稳定性均明显高于游离酶。
关键词: 氨基功能化SBA-15分子筛； 纤维素酶； 固定化
中图分类号： Q 814.2文献标志码： A
Immobilization of Cellulase on
Amino-functionalized SBA-15 Molecular Sieve
LIU Lu-kuan, SU Zhong-liang, LI Lu, YU Shi-tao
(CollegeofChemical Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)
Abstract: Amino-functionalized SBA-15 mesoporous molecular sieve was prepared by the co-condensation method. The immobilization of cellulase was studied using the obtained molecular sieve as carrier and glutaraldehyde as crosslink agent. The effects of factors, such as glutaraldehyde concentration, enzyme dosage, pH and immobilization time, on immobilization were investigated. The properties of immobilized cellulase were also studied. The results showed that the optimum conditions were temperature 60 ℃, pH=4, Km=3.61 mg·mL-1, and the thermal, operational and storage stabilities of immobilized cellulase were better than those of free cellulase.
Key words: amino-functionalized SBA-15 molecular sieve； cellulase； immobilization
收稿日期： 2012-11-26
基金项目: 国家自然科学基金项目（30900126, 31070520）.
作者简介： 刘路宽（1986—），女，硕士研究生.*通信联系人.
