PDF全文下载：2011060626
贺韵雅, 于海峰， 杨蕾
（山东轻工业学院 食品与生物工程学院, 山东 济南 250353）
摘要:采用含有不同浓度NaCl (0，0.2，0.4，0.6，0.8和1.0 mol·L-1) BG11培养基对发状念珠藻进行胁迫处理，处理时间24 h，以此探讨盐胁迫对藻细胞膜脂过氧化和抗氧化物质含量的影响。结果表明：随着NaCl浓度的增大，膜脂氧化程度加剧，细胞膜透性增大，电解质外渗率在1.0 mol·L-1 NaCl下达到了63%，丙二醛（MDA）含量在1.0 mol·L-1 NaCl与对照组相比增加了47%。胞内抗氧化酶系统活性增强，过氧化氢酶 （CAT）、抗坏血酸过氧化物酶（APX）和超氧化物歧化酶（SOD）在0.4~0.6 mol·L-1 NaCl处理下，活性分别达到最大值：0.023 U·mg-1 dw，0.058 U·mg-1 dw和0.085 8 U·mg-1 dw。此外具有自由基清除活性的胞外多糖和脯氨酸含量上升。这一系列抗氧化物质的变化都反映出发状念珠藻在盐胁迫过程种所表现出的积极抗氧化保护机制。
关键词： 盐胁迫； 发状念珠藻； 膜脂过氧化； 抗氧化物质
中图分类号：Q 945.78文献标志码：A
Assessment of Salinity-induced Lipid Peroxidation and Antioxidants of Cyanobacterium Nostoc Flagelliforme
HE Yun-ya, YU Hai-feng, YANG Lei
（School of Food and Bioengineering,Shandong Polytehnic University,Ji’nan 250353,China）
Abstract:Salinity-induced lipid peroxidation and alternations in antioxidants were investigated in cyanobacterium Nostoc flagelliforme, which cultivated with or without different concentrations of NaCl (0.1,0.2,0.4,0.6,0.8,and 1.0 mol·L-1)in BG-11 liquid medium for 24 h. The results indicated a acceleration of lipid peroxidation and membrane permeability,A maximum eletrolyte leakage of 63% was got at 1.0 mol·L-1 NaCl treatment. Compared with control, a increase of 47% in malondialdehyde (MDA) was also got at 1.0 mol·L-1 NaCl treatment.The activities of all studied enzymatic antioxidants were significantly increased by salt stress.The acttivities of catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase(SOD) under 0.4—0.6 mol·L-1 NaCl reached 0.023 U·mg-1 dw，0.058 U·mg-1 dw and 0.085 8 U·mg-1 dw respectively. Moreover，other antioxidants with activity of scavenging free radical including extracelluler polysaccharide and proline were also stimulated under salt stress. Changes in a series of antioxidants indicated an active antioxidative defense metabolism of cyanobacteria N.flagelliforme to salt stress.
Key words: salt stress; Nostoc flagelliforme; lipid peroxidation; antioxidants
收稿日期：2011-09-05
基金项目： 国家自然科学基金项目(20806047);中国博士后科学基金面上资助项目(20090450116); 山东省优秀中青年科学家科研奖励基金项目(BS2011SW029).
作者简介： 贺韵雅(1987—),女，硕士研究生.
