PDF全文下载：2011040372
牛淑妍， 南春彩， 胡志强
（青岛科技大学 化学与分子工程学院，山东 青岛 266042）
摘要: Hg2+可猝灭1,8-蒽二磺酰胺的荧光，故加入GSH后，GSH所带的巯基（—SH）和Hg2+发生络合，夺取了与1,8-蒽二磺酰胺络合的Hg2+，使体系的荧光强度增大，据此可以快速地测定GSH的含量。在pH6.5的Na2HPO4-NaH2PO4缓冲溶液中，1,8-蒽二磺酰胺-Hg2+体系荧光强度的增强值与GSH的浓度在5.0×10-5～5.0×10-4 mol·L-1范围内呈线性关系，检测限为4.16 ×10-6 mol·L-1。
关键词: 谷胱甘肽； 汞离子； 1,8-蒽二磺酰胺； 荧光光谱
中图分类号: O 657.32文献标志码： A
Detection of Glutathione by Mercury Ions Quenching Fluorescence of Sulfamide
NIU Shu-yan, NAN Chun-cai, HU Zhi-qiang
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: The reaction of glutathione(GSH) with Hg2+ and the effect of GSH on the fluorescent systems, i.e. 1,8-anthracene disulfonamide-Hg2+ were studied. Hg2+ can quench the fluorescence of 1, 8-anthracene disulfonamide. With addition of GSH to which the mercapto (—SH) has a firmer combination with Hg2+, GSH would wrested the Hg2+ by combining with 1, 8-anthracene disulfonamide, resulting in the recovery of fluorescence. With this method, GSH was detected rapidly. In the phosphate buffer (pH6.5) solution, a good linear relationship was obtained from 5.0×10-5 to 5.0×10-4 mol·L-1 for GSH, and the detection limit was 4.16×10-6 mol·L-1.
Key words: glutathione; mercury ions; fluorescence intensity; 1,8-anthracene disulfonamide
收稿日期:2010-11-22
基金项目: 国家自然科学基金项目(21075073); 山东省自然科学基金重点项目（ZR2010BZ006）.
作者简介: 牛淑妍（1963—）,女，教授.
