全文下载： 
202404002.pdf
文章编号： 1672-6987（2024）04-0014-09； DOI： 10.16351/j.1672-6987.2024.04.002
任玉东1， 贾志华1， 梁成伟1*， 叶乃好2，3(1.青岛科技大学 海洋科学与生物工程学院，山东 青岛 266042；
2.中国水产科学研究院 黄海水产研究所，山东 青岛 266071；
3.青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室，山东 青岛 266071）
摘要： 评估了30 nm阴离子羧基修饰 (—COOH) 和 100 nm 阳离子氨基修饰 (—NH2)的聚苯乙烯（PS）NPs及其不同浓度下（PS-COOH：0、2、5、8和10 mg·L-1；PS-NH2：0、02、03、04和05 mg·L-1）对海洋微藻微拟球藻（Nannochloropsis oceanica）在生长及光合活性中产生的影响。结果表明，两种NPs在培养基中均出现了聚集和电位绝对值的下降；经过羧基修饰的PS对海洋微拟球藻的生长及光合活性并未产生明显的影响，而经过氨基修饰的PS在不同浓度下对海洋微拟球藻的生长及光合活性产生了不同的影响：低浓度（02 mg·L-1）PS-NH2对海洋微拟球藻生长产生促进作用，高浓度（04和05 mg·L-1）PS-NH2对海洋微拟球藻生长产生抑制作用，海洋微拟球藻在不同浓度PS-NH2胁迫下显示出了低促高抑现象；并且当海洋微拟球藻暴露于02 mg·L-1 PS-NH2时，叶绿素 a（Chl a）含量较对照组高，而其他处理组均低于对照组；此外，在02 mg·L-1 PS-NH2处理下的海洋微拟球藻Fv/Fm值一直高于对照组，而在05 mg·L-1高浓度的PS-NH2胁迫下，海洋微拟球藻的最大光化学效率始终低于对照组，这些结果与生长数据相吻合。
关键词： 表面电荷； 纳米塑料； 聚苯乙烯（PS）； 海洋微拟球藻； 低促高抑； 毒理效应
中图分类号： Q 89文献标志码： A
引用格式： 任玉东， 贾志华， 梁成伟， 等. 不同电荷修饰的聚苯乙烯纳米塑料对海洋微拟球藻（Nannochloropsis oceanica）生长及光合活性的影响［J］. 青岛科技大学学报(自然科学版), 2024, 45（4）： 14-22.
REN Yudong， JIA Zhihua， LIANG Chengwei， et al. Effects of different charged polystyrene nanoplastics on growth and photosynthetic activity of Nannochloropsis oceanica［J］. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2024， 45（4）： 14-22.
Effects of Different Charged Polystyrene Nanoplastics on Growth and
Photosynthetic Activity of Nannochloropsis Oceanica
REN Yudong1， JIA Zhihua1， LIANG Chengwei1， YE Naihao2,3
(1. College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 226071, China;
3. Laboratory of Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and
Technology (Qingdao),Qingdao 226071, China)
Abstract: This study aimed to evaluate effects of 30 nm anionic carboxyl-modified (—COOH) and 100 nm cationic amino-modified (—NH2) polystyrene (PS) NPs at different concentrations (PS-COOH: 0, 2, 5, 8 and 10 mg·L-1; PS-NH2: 0, 02, 03, 04 and 05 mg·L-1) on the growth and photosynthetic activity of the marine microalga N. oceanica. The results showed that the two NPs aggregated and the absolute value of potential decreased in the medium; PS modified with carboxyl group had no obvious effect on the growth and photocombination of N. oceanica, while PS modified with amino groups in different Different concentrations have different effects on the growth and photosynthesis of N. oceanica: low concentrations ( 02 mg·L-1) PS-NH2 can promote the growth of N. oceanica, high concentrations (04， 05 mg·L-1) PS-NH2 can inhibit the growth of N. oceanica, N. oceanica at different concentrations of PS- Under NH2 stress, it showed the phenomenon of low promotion and high inhibition; and when N. oceanica was exposed to 02 mg·L-1 PS-NH2, the content of chlorophyll a (Chl a) was higher than that of the control group, while the other treatment groups were lower than the control group. In addition, the Fv/Fm value of N. oceanica under 02 mg·L-1 PS-NH2 treatment was always higher than that of the control group, while under 05 mg·L-1 high concentration PS-NH2 stress, the maximum photochemical value of N. oceanica Efficiency was consistently lower than in the control group, and these results were consistent with the growth data.
Key words: surface charges; nanoplastics; polystyrene(PS); Nannochloropsis oceanica; hormesis; toxicological effects
收稿日期： 2023-05-12
基金项目： 国家自然科学基金项目（31770393）.
作者简介： 任玉东 (1996—), 男, 硕士研究生.*通信联系人.
