山楂不同部位总黄酮含量与体外美白及抗氧化活性研究

全文下载： 202103005.pdf

文章编号： 1672-6987（2021）03-0027-05； DOI： 10.16351/j.1672-6987.2021.03.005

刘霞， 李照珍， 公亚婷， 姚佳彤， 公衍玲*（青岛科技大学 化工学院，山东 青岛 266042）

摘要： 制备山楂不同部位水提取物和醇提取物，测定总黄酮含量，检测其对酪氨酸酶的抑制率、超氧阴离子自由基清除率和羟自由基清除率。结果表明：山楂醇提取物中总黄酮含量普遍高于水提取物，其中山楂叶高达647%。各提取物均有不同程度的酪氨酸酶抑制、超氧阴离子自由基和羟自由基清除作用。其中，山楂叶和山楂果的水提取物在05 g·mL-1时对酪氨酸酶的抑制率分别达708%、623%；山楂叶提取物清除自由基的能力最强，在05 g·mL-1时，山楂叶水提取物和山楂叶醇提取物对超氧阴离子自由基基的清除率分别为702%和673%；而对羟自由基的清除率分别为783%和891%。本研究为山楂提取物应用于美白抗衰老化妆品提供参考，在实际应用时可根据需要选择山楂不同部位和不同提取溶剂与方法。

关键词： 山楂； 总黄酮； 美白活性； 抗氧化活性

中图分类号： R 932文献标志码： A

引用格式： 刘霞， 李照珍， 公亚婷, 等. 山楂不同部位总黄酮含量与体外美白及抗氧化活性研究［J］. 青岛科技大学学报(自然科学版), 2021, 42(3): 27-31.

LIU Xia, LI Zhaozhen, GONG Yating, et al. Study on the total flavonoids content in different parts of Crataegus pinnatifida and their whitening and antioxidant activities in vitro［J］. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2021, 42(3): 27-31.

Study on the Total Flavonoids Content in Different Parts of Crataegus

Pinnatifida and Their Whitening and Antioxidant Activities in Vitro

LIU Xia, LI Zhaozhen, GONG Yating, YAO Jiatong, GONG Yanling

(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)

Abstract: The water extracts and alcohol extracts from different parts of Crataegus pinnatifida were prepared, the content of total flavonoids was determined, and the inhibition rate of tyrosinase, superoxide anion free radical scavenging rate and hydroxyl free radical scavenging rate were detected. The content of total flavonoids in Crataegus pinnatifida alcohol extract was higher than water extrat, among which the content of Crataegus pinnatifida leaves was as high as 64.7%. All extracts can inhibit tyrosinase, scavenge superoxide anion free radicals and hydroxyl free radicals. Among them, the inhibitory rate on tyrosinase of the water extracts of leaves and fruits reached 708% and 623% at 05 g·mL-1, respectively. The leaves extracts had the strongest scavenging ability on free radicals. At a concentration of 05 g·mL-1, the superoxide anion radical scavenging rates of water extract and alcohol extract of Crataegus pinnatifida leaves were 702% and 673%, while the hydroxyl radical scavenging rates were 783% and 891%, respectively. This study provides a reference for the application of Crataegus pinnatifida extracts in whitening and anti-aging cosmetics. Different parts of hawthorn and different extracting solvents and methods can be selected according to the need in practical application.

Key words: Crataegus pinnatifida； total flavonoid； whitening activity； antioxidant activity

收稿日期： 2020-06-09

基金项目： 山东省重点研发计划项目(2018GSF119005)；青岛科技大学大学生创新创业训练计划项目(2019).

作者简介： 刘霞（1996—），女，硕士研究生.*通信联系人.