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文章编号： 16726987（2019）01001804； DOI： 10.16351/j.16726987.2019.01.002
郭晓艳， 尹文硕， 杨晓燕*， 宋维玲
（青岛科技大学 化学与分子工程学院，山东 青岛 266042）
摘要： 通过结合杂交链式反应（HCR）和荧光共振能量转移（FRET）进行信号放大，构造了一种简便、免酶检测脱氧核糖核酸（DNA）的方法。通过一系列实验验证了该方法实验的可行性，并对杂交链式反应的反应条件进行了优化。在最优条件下检测不同浓度的DNA，计算出检测限为147 nmol·L-1，并且证明了本实验具有良好的选择性，能实现均相中的简便检测。
关键词： 杂交链式反应； 荧光共振能量转移； DNA； 信号放大中图分类号： O 657.3 文献标志码： A引用格式： 郭晓艳， 尹文硕， 杨晓燕， 等. 基于荧光共振能量转移及杂交链式反应检测DNA\[J\]. 青岛科技大学学报（自然科学版）， 2019， 40（1）： 1821.
GUO Xiaoyan, YIN Wenshuo, YANG Xiaoyan, et al. DNA detection technology based on fluorescence resonance energy transfer and hybridization chain reaction amplification\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2019, 40(1)： 1821.
DNA Detection Technology Based on Fluorescence Resonance Energy Transfer and Hybridization Chain Reaction Amplification
GUO Xiaoyan, YIN Wenshuo, YANG Xiaoyan, SONG Weiling
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: A simple, rapid and enzymefree fluorescence biosensor was developed by combining hybridization chain reaction (HCR) for signal amplification and fluorescence resonance energy transfer (FRET) for signal transduction. It was proved that the experiment was feasible with a series of experiments. The reaction time and the reaction temperature of HCR were optimized. The fluorescence signals of DNA with different concentrations were tested under the optimal conditions. The detection limit was 147 nmol·L-1 and it was proved that the experiment had good selectivity. The experiment can also achieve simple detection in homogeneous.
Key words: hybridization chain reaction; fluorescence resonance energy transfer; DNA; signal amplification
收稿日期： 20171228
基金项目： 国家自然科学基金项目（21705086）.
作者简介： 郭晓艳(1992—)，女，硕士研究生.*通信联系人.
