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文章编号： 16726987（2018）06004705； DOI： 10.16351/j.16726987.2018.06.007
王纯a， 柏娟a*， 郑斌b
(青岛科技大学a.体育学院；b.化学与分子工程学院，山东 青岛 266042）
摘要： 以磁性纳米粒子为载体，利用抗原抗体识别作用将探针捕获在磁珠表面，用钴纳米粒子标记抗体为探针，利用鲁米诺CoNPsH2O2直接化学发光体系检测化学发光信号强度，根据化学发光信号强弱实现心肌连接蛋白43含量的测定。心肌连接蛋白43的质量浓度在007~20 ng·mL-1时，方法检测限为003 ng·mL-1。并利用该方法对力竭运动后小鼠心肌连接蛋白43含量进行了测定。
关键词： 心肌连接蛋白43; 钴纳米粒子； 化学发光
中图分类号： O 69文献标志码： A
引用格式： 王纯， 柏娟， 郑斌. 钴纳米粒子直接化学发光体系检测力竭运动小鼠心肌连接蛋白43含量\[J\]. 青岛科技大学学报（自然科学版）， 2018， 39（6）： 4751.
WANG Chun， BAI Juan， ZHENG Bin. Determination of connexin 43 in rats after exhaustive exercise based on cobalt nanoparticles labeling\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2018, 39(6)： 4751.
Determination of Connexin 43 in Rats After Exhaustive Exercise
Based on Cobalt Nanoparticles Labeling
WANG Chuna， BAI Juana， ZHENG Binb
(a.College of Physical Education; b.College of Chemistry and Molecular Engineering, Qingdao University
of Science and Technology, Qingdao 266042, China）
Abstract: A new chemiluminescence method for connexin 43 detection was developed based on cobalt nanoparticles labeling. Firstly, the cobalt nanoparticles labeled antibody was prepared and the connexin 43 antibody was immobilized onto the surface of magnetic bead. Through the recognition of antigensantibody the cobalt labeled abtibody was captured onto the magnetic bead in the presence of connexin 43. Under the optimal experimental condition, the concentration of connexin 43 is linear with electrochemical signals in the range from 007—20 ng·mL-1, the detection limit is 003 ng·mL-1. The method was successfully applied to the determination of connexin 43 in muscle of rats after exhaustive.
Key words: connexin 43; cobalt nanoparticles; chemiluminescence
收稿日期： 20171117
基金项目： 山东省自然科学基金项目（ZR2009BM025）.
作者简介： 王纯(1974—)，女，硕士研究生.*通信联系人.
