基于铕配合物与石墨烯复合材料的ECL免疫传感器

PDF全文下载：2017040019

修宝

（青岛科技大学 化学与分子工程学院，山东 青岛 266042）

摘要： 基于自行制备的Eu(Ⅲ)/氨基化石墨烯复合材料，构建了一种新型的电致化学发光免疫传感器，并应用于人免疫球蛋白的检测。制备复合材料时，二乙基三胺五乙酸（DTPA）作为螯合剂，通过脱水缩合反应连接在二维的氨基化石墨烯片层表面。随之，铕（Eu(Ⅲ)）与DTPA作用，螯合在石墨烯表面。由于铕关键的作用，所得的材料显示出稳定的强烈的电致化学发光性能。配合氨基化石墨烯优越的电化学性能，大的比表面积，良好的分散性，该传感器实现了对人免疫球蛋白的灵敏检测，线性范围是0.5~14 ng·mL^-1，检测限为100 pg·mL^-1。除此之外，该免疫传感器显示出良好的选择性、重现性和稳定性，表现出用于临床检测的良好潜力。

关键词： 电致发光免疫传感器； 氨基石墨烯； 二乙基三胺五乙酸； 铕配合物

中图分类号：  O 614文献标志码： A

引用格式：修宝. 基于铕配合物与石墨烯复合材料的ECL免疫传感器\[J\]. 青岛科技大学学报（自然科学版）， 2017， 38（4）： 19-25.

XIU Bao. ECL immunosensor based on composite of Eu-complex and graphene\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2017, 38(4)： 19-25.

ECL Immunosensor Based on Composite of Eu-complex and Graphene

XIU Bao

(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)

Abstract: In this study, a novel ECL immunosensor was established to detect human immunoglobulin (IgG) as a model target analyte, based on Eu(Ⅲ)/NH2-graphene composites. To build the composite and the sensor, diethylene triamine pentacetate acid (DTPA), as a chelating agent, was bonded to the two-dimensional NH²-graphene sheets, via the dehydration condensation reaction. Eu(Ⅲ) was then chelated to the sheets to form a composite, exhibiting strong and stable cathodic electrochemiluminescence (ECL) activity due to the important roles of Eu(Ⅲ). NH²-graphene has excellent electrochemical properties, large surface area, good dispersion in water, which are good for the immobilization of antibody. The ECL immunosensor was found to give a sensitive response to IgG in a linear range of 0.5-14 ng·mL^-1with a detection limit of 100 pg·mL^-1. Additionally, the proposed immunosensor showed high specificity, good reproducibility, and stability, which might be promising for clinical application.

Key words: ECL immunosensor; amino-graphene; diethylene triamine pentacetate acid (DTPA); europium complex

收稿日期：    2016-03-09

作者简介： 修宝(1987—)，男，硕士研究生.

文章编号： 1672-6987（2017）04-0019-07； DOI： 10.16351/j.1672-6987.2017.04.003