PDF全文下载：2017040040
李昉1,2, 孙鉴昕1
(1.青岛科技大学 化学与分子工程学院，山东 青岛 266042；2.青岛明月海藻集团 海藻活性物质国家重点实验室，山东 青岛 266400）
摘要： 将不同浓度表观遗传修饰剂伏立诺他（SAHA）作用于一株来源于牛蒡叶的黑曲霉，并对激活后发酵液粗提物进行了杀虫抗菌活性测试、活性追踪和GC/MS分析。研究表明：激活后，黑曲霉发酵液粗提物的杀虫活性由激活前的LD50值由0.17 mg·mL-1提高至0.011 mg·mL-1；并且粗提物对多种植物致病菌株均具有活性。对粗提物低极性组分进行GC/MS分析鉴定出了38个化合物，采用柱色谱方法对激活后的发酵液粗提物进行活性跟踪，最佳杀虫活性为LD50=7.304 μg·mL-1。
关键词： 表观遗传； SAHA； 杀虫； 抗菌； 黑曲霉； 牛蒡
中图分类号： Q 939文献标志码： A
引用格式：李昉, 孙鉴昕. 伏立诺他(SAHA)对一株牛蒡内生真菌生物活性物质的激活\[J\]. 青岛科技大学学报（自然科学版）， 2017， 38（4）： 40-46.
LI Fang, SUN Jianxin. Activation on the antibacterial and insecticidal activity in Aspergillus niger derived from Arctium lappa L. by vorinostat (SAHA)\[J\]. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2017, 38(4)： 40-46.
Activation on the Antibacterial and Insecticidal Activity in Aspergillus Niger Derived from Arctium Lappa L. by Vorinostat (SAHA)
LI Fang1,2, SUN Jianxin1
（1.Collage of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; 2.State Key Laboratory of Seaweed Bioactive Compounds,Bright Moon Seaweed Group,Qingdao 266400,China）
Abstract: In this paper, an Aspergillus niger derived from the Arctium lappa L. was activated by different concentration of SAHA, and explored insect resistant and antimicrobial activity of broth extracts. This paper researched the active compounds by bioassay-guided method and with column chromatography and GC/MS. The results showed that after the activation of SAHA, the activities of broth extracts against brine shrimp（LD50） increased from 0.17 mg·mL-1（before activation）to 0.011 mg·mL-1（after activation）,the resistance test against fungi and bacteria showed that after the activation of 0.2 μmol·L-1concentration of SAHA, the crude extract of fermentation inhibited the growth of several plant pathogenic bacteria. In addition, further research of different polar parts of the crude extraction indicated a relatively strong insecticidal toxicity of LD50=7.304 μg·mL-1. The GC/MS data showed that there were 38 compounds in the ether phase and these compounds contained some biologically active metabolite.
Key words: epigenetics; SAHA; insecticidal toxicity; antibacterial; Aspergillus niger; Arctium lappa L.
收稿日期： 2016-01-19
基金项目： 国家自然科学基金项目（41106136）；山东省优秀中青年科学家科研奖励基金项目（BS2012NY002）；青岛市科技计划基础研究项目（1314144-JCH）；地方高校国家级大学生创新创业训练计划项目（201410426053）.
作者简介： 李昉(1975—),女，副教授.
文章编号： 1672-6987（2017）04-0040-07； DOI： 10.16351/j.1672-6987.2017.04.006
