一株螺螨酯降解菌的分离、鉴定和特性研究

PDF全文下载：2016040403

贾海飞， 朱永哲*

（青岛农业大学 化学与药学院，山东 青岛266109）

摘要： 从山东潍坊玉米种植地采集的土壤样品中分离筛选出1株螺螨酯高效降解菌。使用高效液相色谱(HPLC)法测定了分离菌株对螺螨酯、吡虫啉和丁醚脲等8种农药的降解率，对该菌株进行了菌种鉴定并测定了最佳生长条件。结果显示：通过对筛选出的菌株WF146形态学观察及16S rDNA序列分析，初步鉴定该菌株为肠杆菌属（Enterobacter sp.）。确定了该菌株最佳的生长温度为30℃、pH为70。在培养120 h后对螺螨酯的降解率为7642%，对吡虫啉、三氟羧草醚、乙嘧酚磺酸酯、丁醚脲和烯酰吗啉的降解率为2081%~4106%。

关键词： 螺螨酯降解菌； 高效液相色谱法； 肠杆菌属

中图分类号：  X 172文献标志码：A

 Isolation, Identification and Its Characteristics of a Spirodiclofen Degrading Bacterium

 JIA Haifei, ZHU Yongzhe

(College of Chemistry and Pharmaceutical Science, Qingdao Agricultural University, Qingdao 266109,China)

Abstract： A higheffective spirodiclofendegrading bacterium was isolated from corn cultivated soil in Weifang of Shandong. Eight kinds of pesticides（spirodiclofen,imidacloprid and diafenthiuron et al）degradation rate of the strain was detected using HPLC method. The strain identification and the best cultivated conditions were determined. Results: Based on morphology and 16S rDNA gene sequence analysis, the strain was identified as Enterobacter sp. the best cultivated temperature and pH of the strain were 30 ℃ and  70. After 120 h cultivation, the spirodiclofen degradation rate of the screened strain WF146 was 7642%, the degradation rate was 2081%—4106% to imidacloprid, acifluorfene, bupirimate, diafenthiuron and dimethomorph.

Key words: spirodiclofen degrading bacterium; HPLC method; Enterobacter sp.

 收稿日期：20150411

基金项目： 韩国农业振兴厅国际合作研究项目（PJ012094）.

作者简介： 贾海飞（1987—），女，硕士研究生. *通信联系人.

文章编号：16726987（2016）04040305； DOI： 10.16351/j.16726987.2016.04.010