石榴叶抗植物病原菌的活性成分

PDF全文下载：2016030292

孔阳a,马养民b

(陕西科技大学a.食品与生物工程学院；b.化学与化工学院，陕西 西安710021)

摘要：石榴叶60%乙醇提取物用硅胶、sephadexLH20柱色谱等分离纯化，以理化性质和光谱分析鉴定结构。抑菌活性测定采用滤纸片扩散法。石榴叶粗提物对11种植物病原真菌和4种细菌的体外抗菌效果很好，并从中分离纯化得到6种单体化合物，经鉴定结构分别为β谷甾醇（1）、胡萝卜苷（2）、槲皮素3OβD葡萄糖苷（3）、木犀草素3′OβD葡萄糖苷（4）、没食子酸（5）、鞣花酸（6）。化合物（1）~（3）在石榴叶中为首次发现。

关键词： 石榴叶； 植物病原菌； 细菌； 抑菌活性

中图分类号：R 282.71文献标志码：A

Antipathogen Activity Components of Leaves of Punica Granatum L.

KONG Yanga, MA Yangminb

(a.College of Food and Biological Engineering； b.College of Chemisitry and Chemical Engineering，

Shaanxi University of Science and Technology, Xi′an 710021, China)

Abstract: The leaves of Punica granatum L. was extracted with 60 % ethanol, and separated with silica gel and Sephadex LH20 column chromatography. Structures of the compounds were identified by their physical chemical character and spectral analysis. The different extracts of pomegranate leaf total inhibitory against 11 kinds of pathogenesis bacterial and 4 kinds of epiphyte effect is very good. Six compounds were isolated from the leaves of Punica granatum L. They areβsitosterol (1), daucosterol (2), quercetin3OβD glucoside (3), luteolin 3'OβD glucoside (4), gallic acid(5), ellagic acid(6). Compound (1)—(3) were isolated from leaves of Punica granatum L. for the first time.

Key words:  leaves of Punica granatum L.； pathogenesis bacterial; epiphyte; antibacterial activity

收稿日期：20150409

基金项目： 陕西省教育厅科学研究项目计划自然科学专项项目（2013JK0726）.

作者简介： 孔阳(1980—)，女，讲师.

文章编号：16726987（2016）03029205； DOI： 10.16351/j.16726987.2016.03.011