麻黄草微波提取物抑菌效果研究

陈蔚燕1,2，许良忠1*

(1. 青岛科技大学 化学与分子工程学院， 山东 青岛 266042； 2. 青岛农业大学 化学与药学院， 山东 青岛 266109)

 摘要： 以麻黄草为试材，对其微波提取物进行抑菌研究，以抑菌活性为指标，对提取时间、提取功率、溶剂、料液比等因素进行讨论，采用L9(34)正交试验对提取工艺进行优化。采用菌丝生长速率法对各提取物的抑菌活性进行测定，计算出各提取物的半致死浓度，试验数据表明：萃取功率600 W，萃取时间5 min,料液比5∶70，溶剂为丙酮时为最佳提取工艺。

关键词：  麻黄草； 微波； 抑菌

 中图分类号：  TQ 461      文献标志码： A

Research on Antibacterial Activity of Ephedra Extracts by Microwaveassisted

CHEN Weiyan1,2, XU Liangzhong1

(1.College of Chemistry and Molecular Engineering， Qingdao University of Science and Technology, Qingdao 266042， China；

 2. College of Chemistry and Pharmacy, Qingdao Agricultural University, Qingdao  266109， China)

Abstract: This paper investigated the optimum microwaveassisted extraction proeess of ephedra by application of orthogonal tests. Taking the antibacterial activity as index, with extraction time， extraction power，solvent and solidliquid ratio as factors，extraction technology was optimized by L9(34) orthogonal test. By using the mycelium growth rates, the inhibition of ephedra extracts were measured, the minimum inhibitory concentration and EC50 were calculated. It was found that the optimum extraction technology were: microwave power at 600 W, extraction time at 5 min, solidliquid ratio is 5∶70, solvent is acetone.

Key words: Ephedra sinica; microwave; antibacterial

 收稿日期： 20140417

作者简介： 陈蔚燕（1978—），女，博士，讲师.   *通信联系人.

文章编号： 16726987（2015）03028605； DOI： 10.16351/j.16726987.2015.03.011