秦岭药用植物防风内生真菌的分离鉴定及抑菌活性研究

张影珍1， 马养民1,2*， 王鹏飞1， 田从丽3

(1.陕西科技大学 化学与化工学院，教育部轻化工助剂化学与技术重点实验室，陕西 西安 710021;

2.陕西农产品加工技术研究院，陕西 西安 710021; 3.陕西国际商贸学院，陕西 咸阳 712046)

摘要： 采用组织分离法分离新鲜防风中根、茎、叶内的内生真菌，用形态学方法对其进行初步鉴定并使用滤纸片法来测定其次生代谢产物的抑菌活性。从防风中分离得到32株内生真菌，它们分属于7目9科14属，并且具有不同程度的抑菌活性，其中菌种DR12、DL02、DS07和DS09具有较强的抑菌活性。防风内生真菌种类繁多，其次生代谢产物具有广泛的抑菌活性，从防风植物中分离内生真菌尚属首次报道。

 关键词： 防风； 内生真菌； 抑菌活性； 代谢产物

中图分类号： O 629           文献标志码：  A

 Isolation, Identification and Antimicrobial Activities of Endophytic Fungi from Saposhnikovia Divaricata

ZHANG Yingzhen1, MA Yangmin1,2, WANG Pengfei1, TIAN Congli3

(1.College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology,

Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry,Ministry of Education, Xi′an  710021, China;

 2.Shaanxi Research Institute of Agricultural Products Processing Technology,Xi′an  710021, China;

3.Shaanxi Institute of International Trade and Commerce,Xianyang 712046, China)

Abstract： Endophytic fungi were isolated from the root,stem and leaf of Saposhnikovia divaricate, and then they were morphologically identified. The antimicrobial activities were tested by paper sheet method. 32 strains of endophytic fungi isolated from Saposhnikovia divaricate were classified into 7 orders, 9 families and 14 genus. Almost 32 strains presented antimicrobial activities. DR12, DL02, DS07 and DS09 show  a strong antimicrobial activity especially. There are abundant and multiple endophytic fungi in Saposhnikovia divaricate and their secondary metabolites have a wide range of antimicrobial activity.The endophytic fungi of Saposhnikovia divaricate were firstly reported in this paper.

Key words: Saposhnikovia divaricate; endophytic fungi; antimicrobial active; secondary metabolites

 收稿日期： 20140526

基金项目: 高等学校博士学科点专项科研基金项目（20116125110001）;陕西省自然科学基础研究计划项目（2014JZ003）.

作者简介： 张影珍(1989—)，女，硕士研究生.             *通信联系人.