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Cu2+猝灭茜素红荧光法检测谷胱甘肽

作者:时间:2014-07-15点击数:

PDF全文下载:2014030221

牛淑妍, 沈晓彤, 崔欢欢, 孙娟

 (青岛科技大学 化学与分子工程学院,山东 青岛 266042)

 摘要: 茜素红(ARS)与硼酸(H3BO3)之间能发生相互作用形成黄色配合物,使ARS 的荧光增强,Cu2+可以猝灭茜素红在BrittonRobison (BR)缓冲溶液中的荧光。加入谷胱甘肽(GSH)后,由于GSH所带的巯基(—SH)和Cu2+发生络合,使体系的荧光强度增大,据此可以快速的测定GSH的含量。在pH 6.3的BR缓冲溶液中,茜素红Cu2+体系荧光强度的增强值与GSH的浓度在1.0×10-5~1.9×10-4 mol·L-1范围内呈线性关系,检测限为1.0×10-5 mol·L-1。

 关键词: 谷胱甘肽;铜离子;茜素红;荧光光谱

 中图分类号:O 657.32文献标志码:A

 Detection of Glutathione by Copper Ions Quenching Fluorescence of Alizarin Red

 NIU Shuyan, SHEN Xiaotong, CUI Huanhuan, SUN Juan

 (CollegeofChemistryand Molecular Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)

 Abstract: The alizarin red (ARS) interacted with H3BO3 to form a yellow complex, which resulted in the fluorescence enhancement of the ARS. Cu2+ could quench the fluorescence of ARS. The reaction of glutathione (GSH) with Cu2+ and the effect of GSH on the fluorescent systems, i.e. ARSCu2+ were studied. The mercapto (SH) of GSH added into the system had a firmer combination with Cu2+, which would wrested the Cu2+ combined with H3BO3, resulting in the recovery of fluorescence. As a result, GSH was detected rapidly. In the BrittonRobison buffer (pH 63) solution, a good linear relationship was obtained from 10×10-5 to 19×10-4 mol·L-1 for GSH, the detection limit was 10×10-5 mol·L-1.

 Key words: glutathione; copper ions; alizarin red; fluorescence spectrum

收稿日期: 20130327

基金项目: 国家自然科学基金项目(21075073);山东省自然科学基金重点项目(ZR2010BZ006).

作者简介: 牛淑妍(1963-),女,教授.

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