Cu2+猝灭茜素红荧光法检测谷胱甘肽

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牛淑妍， 沈晓彤， 崔欢欢， 孙娟

 （青岛科技大学 化学与分子工程学院，山东 青岛 266042）

 摘要： 茜素红（ARS）与硼酸（H3BO3）之间能发生相互作用形成黄色配合物，使ARS 的荧光增强，Cu2+可以猝灭茜素红在BrittonRobison （BR）缓冲溶液中的荧光。加入谷胱甘肽（GSH）后，由于GSH所带的巯基（—SH）和Cu2+发生络合，使体系的荧光强度增大，据此可以快速的测定GSH的含量。在pH 6.3的BR缓冲溶液中，茜素红Cu2+体系荧光强度的增强值与GSH的浓度在1.0×10-5～1.9×10-4 mol·L-1范围内呈线性关系，检测限为1.0×10-5 mol·L-1。

 关键词： 谷胱甘肽；铜离子；茜素红；荧光光谱

 中图分类号：O 657.32文献标志码：A

 Detection of Glutathione by Copper Ions Quenching Fluorescence of Alizarin Red

 NIU Shuyan, SHEN Xiaotong, CUI Huanhuan, SUN Juan

 (CollegeofChemistryand Molecular Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)

 Abstract: The alizarin red (ARS) interacted with H3BO3 to form a yellow complex, which resulted in the fluorescence enhancement of the ARS. Cu2+ could quench the fluorescence of ARS. The reaction of glutathione (GSH) with Cu2+ and the effect of GSH on the fluorescent systems, i.e. ARSCu2+ were studied. The mercapto (SH) of GSH added into the system had a firmer combination with Cu2+, which would wrested the Cu2+ combined with H3BO3, resulting in the recovery of fluorescence. As a result, GSH was detected rapidly. In the BrittonRobison buffer (pH 63) solution, a good linear relationship was obtained from 10×10-5 to 19×10-4 mol·L-1 for GSH, the detection limit was 10×10-5 mol·L-1.

 Key words: glutathione; copper ions; alizarin red; fluorescence spectrum

收稿日期： 20130327

基金项目： 国家自然科学基金项目(21075073)；山东省自然科学基金重点项目（ZR2010BZ006）.

作者简介： 牛淑妍(1963-)，女，教授.