全文下载:2013060555
牛淑妍, 刘洪霞, 孙娟
(青岛科技大学 化学与分子工程学院,山东 青岛 266042)
摘要: 建立了乙二醛作为荧光衍生剂测定多巴胺(DA)的新方法。DA与乙二醛反应生成一种强荧光物质,其最大激发波长是410 nm,最大发射波长是500 nm。DA的浓度在1.0×10-7~1.0×10-5 mol·L-1范围内与荧光强度呈线性关系,线性方程ΔF=1.24 cDA/10-7 mol·L-1+0.47,线性相关系数为γ=0.999 2,检出限为4.27×10-8 mol·L-1。优化了实验条件(缓冲溶液种类、pH、反应时间和乙二醛的用量),考察了干扰离子等对测定的影响,此法可用于注射液中DA含量的测定。
关键词: 多巴胺;乙二醛;荧光光度法
中图分类号: O 657. 32文献标志码: A
Fluorimetric Study on the Derivatives by Reaction of Dopamine with Glyoxal
NIU Shu-yan, LIU Hong-xia, SUN Juan
(CollegeofChemistryand Molecular Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)
Abstract: A novel method for the detection of dopamine (DA) using glyoxal as the fluorescent derivatives reagent is described. DA reacts with glyoxal to form a compound which was a strong fluorescent. The measurement was carried out at λem 500 nm with excitation at λex 410 nm.A good linear relationship was obtained from 1.0×10-7—1.0×10-5mol·L-1 for DA, the detection limit was 4.27×10-8mol·L-1. The calibration equation is ΔF=1.24 cDA/10-7mol·L-1+0.47, with a correlation coefficient of 0.999 2. The experiment condition (buffer solution, pH, reaction time, dosage of glyoxal) were optimized, and foreign ions on the determination of DA were examined. The method can be used for the determination of DA in injection samples.
Key words: dopamine; glyoxal; fluorescence spectroscopy
收稿日期:2012-12-04
基金项目: 国家自然科学基金项目(21075073); 山东省自然科学基金重点项目(ZR2010BZ006).
作者简介: 牛淑妍(1963─),女,教授.