全文下载：2013010042
樊曰伟, 张如敏, 袁瑾*
(青岛科技大学 化学与分子工程学院，山东 青岛 266042)
摘要： 利用DNA 自组装原理合成了一种高灵敏的线形一体化细胞探针，用于快速、高灵敏以及高特异性的癌细胞定量分析。该探针采用了功能元件一体化的设计，以DNA 纳米线为载体，将磁分离元件、识别元件以及信号元件集中于一体。该探针与电化学检测手段相结合，实现了对人B淋巴瘤细胞（Ramos 细胞）的快速定量分析，检测时间仅需30 min，检测限可达到200 个细胞。通过选择性实验证明了该方法不仅具有高的灵敏度，还具有良好的特异性，因此，具有潜在的应用价值。
关键词： 癌细胞探针； DNA； 适体； 溶出伏安法
中图分类号： O 657.1;Q 523文献标志码： A
A Sensitive All-in-One Linear-Shaped Probe for the Rapid Detection of Cancer Cells
FAN Yue-wei, ZHANG Ru-min, YUAN Jin
(CollegeofChemistryand Molecular Engineering,QingdaoUniversityof Science and Technology,Qingdao266042,China)
Abstract: An all-in-one linear-shaped cell probe was prepared via the DNA self-assembly principles, which was more quickly, sensitively and specifically to quantitative detect cancer cells. DNA nanowire complexed with the detection unit, magnetic separation unit and the signal unit, was used to construct the all-in-on probe. With the electrochemical detection method, we can quickly quantitative detect B lymphoma cells within 30 minutes, and the detection limit is 200 cells. Selective experiments prove that the method not only has high sensitivity but also good specificity, which has a good potential application.
Key words: cancer cell probe； DNA; aptamer; stripping voltammetry
收稿日期：2012-03-23
基金项目： 国家自然科学基金项目（21025523）.
作者简介： 樊曰伟(1986—）,男，硕士研究生. *通信联系人.
