全文下载：2012040372
陈云龙, 王明慧*
(青岛科技大学 化学与分子工程学院， 山东 青岛 266042)
摘要: 合成了一种新型一氧化氮分子荧光探针并对其结构进行了表征，此荧光探针为连接有1,4,8,11-四氮杂环十四烷环胺化合物的荧光素-环胺-Cu(II)离子配合物，其本身无荧光，当与NO反应后，在λex=465 nm光激发下产生荧光且最大发射波长λem =524 nm。体系的pH值对荧光强度有影响，在pH为4.5时荧光强度最大。在H2O2，ONOO-存在下，荧光探针对NO表现出很好的选择性。以NOC-18为NO释放剂，在NOC-18浓度为4×10-5~14×10-5mol·L-1范围内建立了NOC-18浓度和荧光强度间的关系曲线，荧光强度随NOC-18浓度的增加而增大，可实现对NO的直接检测。
关键词: 荧光探针； 一氧化氮； 荧光素-环胺-Cu(II)离子配合物； 1,4,8,11-四氮杂环十四烷环胺
中图分类号: O 656.3 文献标志码： A
Synthesis of Fluorescein-based Copper Fluorescent Probe and Its Application in NO Detection
CHEN Yun-long, WANG Ming-hui
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: A novel fluorescent probe for NO, fluorescein-Cu(II) complex connecting with 1,4,8,11-tetraazacyclotetra-decane, was synthesized and characterized. The complex himself showed non-fluorescent, when reacted with NO, it showed a strong fluorescent peak at λem =524 nm and λex=465 nm. The pH could affect fluorescence intensity and there was a maximum value when pH=4.5. The fluorescent probe exhibited an excellent selectivity to NO in the presence of H2O2 or ONOO-. A relationship curve was developed between fluorescent intensity and concentration of NOC-18 (a donor of NO). At range of 4×10-5 to 14×10-5 mol·L-1 of NOC-18, the fluorescent intensity was increased with increasing of NOC-18 concentration. Therefore, NO can be determined directly through measuring fluorescent intensity.
Key words: fluorescent probe; nitrogen monoxide; fluorescein-Cu(II) complex; 1,4,8,11-tetraaza-cyclotetradecane
收稿日期：2011-10-19
基金项目： 山东省自然科学基金项目（ZR2009BM019）.
作者简介： 陈云龙(1987—),男，硕士研究生.*通信联系人.
