全文下载：2012030263
杜晓梅, 付国家, 郭庆杰*, 王许云
（青岛科技大学 化工学院； 山东省清洁化工过程重点实验室, 山东 青岛 266042）
摘要: 以啤酒糟为主要原料，采用黑曲霉3.316(Aspergillus niger 3.316)固态发酵生产纤维素酶，对培养基组成和培养条件进行优化。结果表明：啤酒糟和麸皮的质量比为8∶2、水料质量比为1.5∶1、硫酸铵质量分数为3%、发酵时间为4 d时，所产纤维素酶活性最大，滤纸酶活性(FPA)和羧甲基纤维素酶活性(CMCA)分别达72.611 8和692.170 0 nkat。
关键词: 啤酒糟； 黑曲霉； 固态发酵； 纤维素酶
中图分类号: Q 936文献标志码： A
Cellulase Production from Brewer’s Spent Grain by Solid-State Fermentation with Aspergillus Niger 3.316
DU Xiao-mei, FU Guo-jia, GUO Qing-jie, WANG Xu-yun
(College of Chemical Engineering； Key Laboratory of Clean Chemical Processing Engineering ofShandongProvince,
QingdaoUniversityof Science and TechnologyQingdao266042,China)
Abstract: Cellulase production was carried out by Solid-State Fermentation (SSF) with Aspergillus niger 3.316 using brewer’s spent grain as the main material. The SSF conditions were studied and optimized. It indicated that the optimum conditions were as follows: the ratio of brewer’s spent grain and wheat bran was 8∶2, the ratio of water to material was 1.5∶1, the content of the ammonium sulfate was 3%, and the fermentation time was 4 d, respectively. Under the above-mentioned conditions, the filter paper activity (FPA) and carboxymethyl cellulase activity (CMCA) can reach 72.611 8 and 692.170 0 nkat, respectively.
Key words: brewer’s spent grain; Aspergillusniger; solid-state fermentation; cellulase
收稿日期：2011-12-23
基金项目： 山东省科技攻关项目(2009GG10007001)；山东省自然科学杰出青年基金项目(JQ200904).
作者简介： 杜晓梅(1986—)，女，硕士研究生. *通信联系人.
