全文下载：2012010038
秦胜利1， 王建广1，2
（1.青岛科技大学 化工学院，山东 青岛 266042； 2.山东出入境检验检疫局，山东 青岛 266002）
摘要: 采用自行建立和优化的依赖于核酸序列恒温扩增（NASBA）检测体系，对溶藻弧菌进行检测。采用溶藻弧菌的hsp60基因为目的片段设计特异性引物，建成可快速检测溶藻弧菌的NASBA检测法，并进行了特异性和灵敏度试验。结果表明：所建立起的NASBA检测方法，灵敏度为6.9×102 cfu·mL-1，高于普通PCR方法。溶藻弧菌的依赖于核酸序列恒温扩增检测方法具有较高灵敏度和和良好特异性，并且对仪器要求更低，用普通恒温设备即可进行反应，具有广阔的推广前景。
关键词: 溶藻弧菌; 检测; 依赖于核酸序列恒温扩增
中图分类号: Q 52文献标志码： A
Establishment of the Nucleic Acid Sequence-based Amplification Method for Detecting Vibio Alginolyticus
QIN Sheng-li1, WANG Jian-guang1,2
（1.College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042，China；2. Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China）
Abstract: A new method, based on Nucleic Acid Sequence-based Amplification（NASBA） to detect Vibio alginolyticus of samples, was established. A highly specific set of primers was synthesized to target the hsp60 gene of Vibio alginolyticus so as to establish Nucleic Acid Sequence-based Amplification method. Specificity and sensitivity were tested.The results showed that the sensitivity of NASBA was 6.9×102 cfu·mL-1 which was higher than the result of PCR method. Detecting Vibio alginolyticus with NASBA was more specific and sensitive than PCR method and has lower instrumental requirement. So,there is a broad prospect.
Key words: Vibio alginolyticus； detection； Nucleic Acid Sequence-based Amplification
收稿日期:2011-05-10
基金项目: 国家质检总局科研项目（2008IK140）；山东出入境检验检疫局科研项目（SK200821）.
作者简介: 秦胜利（1979—），男，硕士研究生.
