PDF全文下载：2011060605
王春凤， 周国伟*， 李天铎， 许云强， 宋宏斌， 张磊
（山东轻工业学院 山东省轻工助剂重点实验室；材料科学与工程学院，山东 济南250353）
摘要: 以三嵌段聚合物(P123)和表面活性剂聚乙二醇(PEG 4000)为复合模板剂，使用水热法合成了高度有序的介孔SiO2 (MS)，并用3-氨丙基三乙氧基硅烷(APTES)对其进行后接枝改性。通过XRD、SEM、TEM和氮气吸脱附等手段对介孔SiO2进行了表征。结果表明，该介孔材料具有高度有序的六方状介孔结构。当介孔材料经表面氨基改性后，其孔径、孔体积和比表面积均相应降低，但其形貌和介孔结构仍保持不变。以该材料为载体对假丝酵母脂肪酶(CRL)固定化，考察了改性前后酶固定量和固定化酶的催化活性。结果显示改性后介孔SiO2固定化酶具有较好的催化活性和重复利用性，重复利用5次后，该固定化酶仍具有其最初活性的50%以上。
关键词: 介孔二氧化硅； 氨基改性； 假丝酵母脂肪酶； 固定化
中图分类号: Q 814.2文献标志码： A
Immobilization of Lipase on Amino-functionalized Mesoporous Silica Prepared Using a Composite Template
WANG Chun-feng, ZHOU Guo-wei , LI Tian-duo, XU Yun-qiang， SONG Hong-bin, ZHANG Lei
(Shandong Provincial Key Laboratory of Fine Chemicals, School of Material Science
and Engineering, Shandong Polytechnic University, Ji’nan 250353, China)
Abstract: Mesoporous silica (MS) was synthesized via hydrothermal process using triblock copolymer Pluronic P123 and poly (ethylene glycol) (PEG 4000) as dual templates. The materials were functionalized with (3-aminopropyl)triethoxysilane APTES by post-synthesis-grafting method. The results indicated that mesoporous silica possessed a 2-dimensional hexagonal mesoscopic structure. The mesoscopic structure could be remained, while pore diameters, pore volumes, and surface areas were decreasing after the functionalization. Candida rugosa lipase (CRL) was used as a model enzyme for studying the effect of amino-functionalized in loading amount and enzymatic activity. As a result, APTES functionalized mesoporous silica immobilized CRL showed higher hydrothermal stability and the greatest reusability, and its residual activity was maintained at levels of 50% to the initial value after the fifth reuses.
Key words: mesoporous silica; amino-functionalization; Candida rugosa lipase; immobilization
收稿日期:2011-09-25
基金项目： 国家自然科学基金项目(20976100)；山东省自然科学基金项目(ZR2010BM013)；山东省科技发展计划项目(2010GGX10306).
作者简介: 王春凤(1986—)，女，硕士研究生. *通信联系人.
