PDF全文下载：2011050488
许泳吉， 王永智
（青岛科技大学 化学与分子工程学院，山东 青岛 266042）
摘要: 设计并合成了4种相对分子质量较小、疏水性较低的荧光衍生试剂：邻氨基苯甲酸酐、N-甲基邻氨基苯甲酸酐、2-氨基烟酸活性酯和水杨酸活性酯。系统研究了这些衍生试剂与己胺的反应性能，并合成了相应的衍生物标准品，测定了衍生物的荧光性质。结果表明：4种衍生试剂中邻氨基苯甲酸酐适合用作高效液相色谱荧光检测多胺的衍生试剂。
关键词: 高效液相色谱； 荧光衍生试剂； 多胺
中图分类号:O 657.7文献标志码： A
Synthesis and Properties of Four Fluorescent Reagents for
HPLC Determination of Polyamines
XU Yong-ji， WANG Yong-zhi
(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)
Abstract: Four fluorescent reagents, anthranilic acid anhydride, N-methyl-o-aminobenzoic acid anhydride, 2-aminonicotinic acid active ester and salicylic acid active ester, were designed and synthesized. The reaction properties between these reagents and hexylamine were studied. The corresponding standard derivatives were synthesized and the fluorescent properties of these derivatives were also measured. The results showed that anthranilic acid anhydride was a suitable fluorescent reagent for detection of polyamine by HPLC.
Key words: high performance liquid chromatography (HPLC); fluorescent reagents; polyamine
收稿日期:2011-03-22
作者简介: 许泳吉（1960—）,男,教授.
