PDF全文下载：2011020124
李霞， 张青瑞*， 曹长青， 姜桢
（青岛科技大学 化工学院，山东 青岛 266042）
摘要: 利用Swiss-Model建立了克雷伯氏杆菌中FNR蛋白完整三级结构。模型的可靠性经Ramachandran图进行验证，分析发现模建蛋白85.8%残基φ和ψ角落在最佳区域，12.8%的残基落入其他许可区，0.5%的残基落在勉强许可区，0.9%的残基落在不允许区。经Scanprosite服务器对FNR蛋白功能域和活性位点进行搜索发现，FNR主要包括环核苷酸结合域和螺旋-转角-螺旋DNA结合域两个区域。同时对FNR蛋白中\[4Fe-4S\]簇结构的合理位置进行了探讨。
关键词: 克雷伯氏杆菌； FNR蛋白； \[4Fe-4S\]簇
中图分类号: O 359 文献标志码： A
Modeling the Tertiary Structure of FNR Oxygen Regulatory Protein in Klebsiella Pneumoniae
LI Xia， ZHANG Qing-rui， CAO Chang-qing， JIANG Zhen
(College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042，China)Abstract: The tertiary structure of FNR protein was built by Swiss-Model. Reliability of the model was validated by the Ramachandran plot, which showed that φ and ψ angles of residues in most favoured regions were 85.8%, residues in additional allowed regions were 12.8%, residues in generously allowed regions were 0.5% and residues in disallowed regions were 0.9%, respectively. The domain and active sites of FNR were searched by ScanProsite Server. It was shown that FNR contained cyclic nucleotide-binding domain and the helix-turn-helix DNA binding domain. The reasonable position of \[4Fe-4S\] cluster was discussed.
Key words: Klebsiella pneumoniae; FNR protein; \[4Fe-4S\] clusters
收稿日期：2010-11-26
作者简介：李霞（1986—）,女，硕士研究生. *通信联系人.
