PDF全文下载：2011010038
田智刚1， 李红芳2， 梁生康3， 李俊峰2*
(1．晋中市环保局 榆次区分局，山西 晋中 030600；2．青岛科技大学 化工学院，山东 青岛 266042；3．中国海洋大学 化学化工学院，山东 青岛 266003)
摘要：从胜利油田附近石油污染土壤采集土样，以菲为唯一碳源的选择性培养基分离筛选到一株菲高效降解菌SLY-3，根据形态、生理生化特性及16S rDNA比对初步鉴定该菌株为芽孢杆菌属，并对其降解菲的特性进行了研究。结果表明，菌株SLY-3在菲浓度为80 mg·L-1条件下，28 ℃振荡培养96 h，对菲的降解率达到93.73％；在菲浓度为200 mg·L-1时，培养96 h后的菲降解率为98.36％。同时发现菲的降解程度与细菌数量的增长呈正相关关系。
关键词： 菲降解； 多环芳烃； 芽孢杆菌
中图分类号： Q 93； X 172 文献标志码： A
Screening of a Phenanthrene-degrading Bacterium and Its Characteristics
TIAN Zhi-gang1, LI Hong-fang2, LIANG Sheng-kang3, LI Jun-feng 2
(1.Yuci Substation, Jinzhong Environmental Protection Bureau, Jinzhong 030600, China; 2.College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; 3. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China)
Abstract: Several PAHs-degrading bacteria were isolated from the soil polluted by oil near Shengli Oilfield with selective medium using phenanthrene as the sole carbon source. One high phenanthrene-degrading strain SLY-3 was chosen and identified as Bacillus sp. by physiological and biochemical characteristics and 16S rDNA. The study on the phenanthrene-degrading characteristics of the strain showed that at 28 ℃, the degradation rate of phenanthrene (80 mg·L-1) was 93.73% after cultured 96 h. The degradation rate of phenanthrene (200 mg·L-1) was 98.36% after 96 h rotary culture. A significant positive relationship was found between bacterial growth and phenanthrene degradation.
Key words: phenanthrene-degrading; polycyclic aromatic hydrocarbons; Bacillus sp.
收稿日期：2010-09-03
基金项目： 中国烟草总公司山东省公司科技计划项目(KN172).
作者简介： 田智刚(1971—)，男，工程师. *通信联系人.
