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基于三维DNA分子机器信号放大比率型拉曼光谱检测细胞内miRNA

作者:时间:2020-07-28点击数:

全文下载:    202004002.pdf


文章编号: 1672-6987202004-0020-07 DOI 10.16351/j.1672-6987.2020.04.002

 

毕成, 牛淑妍, 何鹏*

(青岛科技大学 化学与分子工程学院,山东 青岛 266042)

 

摘要: 构建了比率型-拉曼光谱3D DNA Walker纳米分子放大机器对肿瘤细胞标志物miRNA-21及肿瘤细胞进行检测。由miRNA-21作为3D DNA Walker的激活剂,其运动轨迹依赖于金纳米粒子的DNA高负载。3D DNA Walker触发后,由于DNA的循环信号放大作用,实现对miRNA-21的比率型拉曼光谱检测。比率型分析方法解决了分析条件的时空变化引起的信号波动,具有更高的灵敏度和可信度,此方法还可应用到肿瘤细胞的成像研究。

关键词: MicroRNAs 肿瘤细胞; DNA分子机器; 双模式检测

中图分类号: O 657.3文献标志码: A

引用格式: 毕成, 牛淑妍, 何鹏. 基于三维DNA分子机器信号放大比率型拉曼光谱检测细胞内miRNA\[J\]. 青岛科技大学学报(自然科学版), 2020 414): 20-26.

BI Cheng NIU Shuyan, HE Peng.  A ratiometric SERS biosensor for miRNA detection based on three-dimensional DNA molecular machine\[J\]. Journal of Qingdao University of Science and TechnologyNatural Science Edition), 2020 414): 20-26.

 


A Ratiometric SERS Biosensor for miRNA Detection Based on

 

Three-dimensional DNA Molecular Machine BI Cheng NIU Shuyan HE Peng

(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China)

 

Abstract: A ratiometric SERS nanosensing platform was fabricated for detection of miRNA coupling with a DNA tetrahedron-assisted 3D DNA Walker. The proposed nanomachine is actuated by target miRNA binding to blocker DNA. The autonomous and progressive walk on the DNA track via the intramolecular toehold-mediated strand displacement amplification. This method not only effectively avoided the false positive signal, but also proposed a new ratiometric method. The novel ratiometric strategy exhibited excellent specificity and sensitivity. Moreover, the nanomachine was applied for miRNA imaging inside living cells.

Key words: MicroRNAs; tumor cell; DNA molecular machine; dual-mode detectionMicroRNAs



收稿日期:  2019-11-04

基金项目: 国家自然科学基金项目(21505082 21705086);山东省高校科技计划项目(J16LC10.

作者简介: 毕成(1995), , 硕士研究生.*通信联系人.


 

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