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文章编号： 1672-6987（2024）06-0013-08； DOI： 10.16351/j.1672-6987.2024.06.002
曾茵娟1,2， 于坚铜2， 王雪瑜莺2， 王一凡2， 李祥博2， 公衍玲2*（1.广东省妇幼保健院，广东 广州 511442； 2.青岛科技大学 化工学院，山东 青岛 266042）
摘要： 本研究旨在对比罗马洋甘菊精油和德国洋甘菊精油的体外抗过敏、美白、抑菌和对细胞活力和细胞迁移的影响。通过测定透明质酸酶抑制率评价体外抗过敏活性。通过测定酪氨酸酶抑制率评价体外美白活性。采用抑菌圈法测定精油对金黄色葡萄球菌、表皮葡萄球菌和绿脓杆菌的抑制作用。采用MTT法检测人脐静脉内皮细胞（HUVEC）和人皮肤成纤维细胞（HSF）的活力。采用细胞划痕检测细胞迁移。结果表明，两种精油均可以显著抑制透明质酸酶和酪氨酸酶活性，具有美白和抗过敏活性，且表现出剂量依赖关系。洋甘菊精油可用于制备美白舒敏化妆品，其中罗马洋甘菊精油的作用优于德国洋甘菊精油。同时，两种精油还可不同程度地抑制金黄色葡萄球菌、表皮葡萄球菌和绿脓杆菌，且德国洋甘菊精油比罗马洋甘菊精油抑菌作用更强。两种精油可显著提高HUVEC和HSF细胞活力，促进细胞迁移，呈剂量依赖性。洋甘菊精油可用于促进伤口愈合，其中，德国洋甘菊精油的作用优于罗马洋甘菊。德国洋甘菊精油和罗马洋甘菊精油均具有抗过敏、美白、抑菌活性和促进伤口愈合的作用，可根据不同的应用开发目的选择不同精油。
关键词： 洋甘菊； 精油； 抗过敏； 美白； 伤口愈合
中图分类号： R 932文献标志码： A
引用格式： 曾茵娟， 于坚铜， 王雪瑜莺， 等. 洋甘菊精油体外活性的对比研究［J］. 青岛科技大学学报(自然科学版), 2024, 45（6）： 13-20.
ZENG Yinjuan, YU Jiantong, WANG Xueyuying, et al. Comparative study on the activity of chamomile essential oil in Vitro［J］. Journal of Qingdao University of Science and Technology（Natural Science Edition), 2024， 45（6）： 13-20.
Comparative Study on the Activity of Chamomile Essential Oil in Vitro
ZENG Yinjuan1,2, YU Jiantong2, WANG Xueyuying2, WANG Yifan2, LI Xiangbo2, GONG Yanling2
(1.Guangdong Women and Children Hospital, Guangzhou 511442, China;
2.College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042,China)
Abstract: The aim of this study was to compare the antiallergic, whitening, antibacterial and effects on cell viability and migration of Roman chamomile essential oils and German chamomile essential oils in vitro. The in vitro antiallergic activity was evaluated by measuring the inhibition rate of hyaluronidase. The in vitro whitening activity was evaluated by measuring tyrosinase inhibition rate. The inhibitory effects of essential oil on Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa were determined by bacteriostatic zone method. The viability of Human Umbilical Vein Endothelial Cells (HUVEC) and Human Skin Fibroblast (HSF) was measured by MTT assay. Cell migration was detected by cell scratches assay. The results showed that the two essential oils could significantly inhibit the activities of hyaluronidase and tyrosinase, and had whitening and anti-allergic activities with a dose-dependent relationship. Chamomile essential oil can be used in the preparation of whitening and soothing cosmetics, and the effect of Roman chamomile essential oil is better than that of German chamomile essential oil. At the same time, the two essential oils can inhibit Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa to varying degrees, and German chamomile essential oil has stronger antibacterial effect than Roman chamomile essential oil. The two essential oils significantly increased HUVEC and HSF cell viability and promoted cell migration in a dose-dependent manner. Chamomile essential oil can be used to promote wound healing, of which German chamomile essential oil is superior to Roman chamomile. German chamomile essential oil and Roman chamomile essential oil have anti-allergy, whitening, antibacterial activity and promote wound healing. Different essential oils can be selected according to different application and development purposes.
Key words: chamomile; essential oil; antianaphylaxis; whitening; promote wound healing
收稿日期： 2023-11-21
基金项目： 山东省自然科学基金项目（ZR2020MH055）.
作者简介： 曾茵娟（1987—），女，硕士研究生.*通信联系人.
