PDF全文下载：2011020128
胡孔诚1， 朱英杰1， 吕庆春2， 刘艳辉2， 兰东晓1
（1. 青岛科技大学 化学与分子工程学院，山东 青岛 266042；
2. 国家海洋局 天津海水淡化与综合利用研究所，天津 300192）
摘要: 研制了基于纳米多孔金（NPG）电极的电化学DNA传感器。首先用脱合金法制备多孔纳米金（NPG），然后将其修饰到玻碳电极上制备NPG电极。在该电极上固定捕获DNA，与靶DNA杂交后，以邻菲咯啉钴为指示剂，用脉冲伏安法对目标DNA进行检测。DNA/NPG传感器与裸金电极制备的同类型传感器相比，具有更高的灵敏度。并对DNA/NPG传感器的选择性和再生性进行了研究。
关键词: DNA传感器； NPG电极； 修饰电极
中图分类号:O 657.1 文献标志码： A
Preparation of DNA Electrochemical Sensor Based on NPG Electrodes
HU Kong-cheng1, ZHU Ying-jie1, L Qing-chun2, LIU Yan-hui2, LAN Dong-xiao1
(1.College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China；
2.Tianjin Institute of Seawater Desalination and Comprehensive Utilization,State Oceanic Administration,Tianjin 300192,China)
Abstract: Electrochemical DNA sensor based on the nanoparticles porous gold (NPG) electrode was prepared. Firstly NPG was prepared using a dissolved alloy method.The product was modified with a glassy carbon electrode.DNA was fixed and captured in the electrode.After target DNA hybridization, with the \[Co(phen)33+\] as an indicator, pulse voltammetry was used to detect the target DNA.Results showed that NPG electrochemical DNA sensor has a higher sensitivity than gold electrode sensor,and it also has a good selectivity and reproducibility.
Key words: DNA sensor; NPG electrode; modified electrode
收稿日期：2010-11-09
基金项目：国家自然科学基金项目（21075072）；生命分析化学教育部重点实验室开放课题（KLACLS 08015）.
作者简介： 胡孔诚（1968—），男，副教授.
